Applying Tissue Slice Culture in Cancer Research-Insights from Preclinical Proton Radiotherapy

被引：19

作者：

Suckert, Theresa ^{[1
,2
,3
,4
]}

Rassamegevanon, Treewut ^{[1
,2
,3
,4
]}

Mueller, Johannes ^{[3
,4
,5
]}

Dietrich, Antje ^{[1
,2
,3
,4
]}

Graja, Antonia ^{[1
,2
,3
,4
]}

Reiche, Michael ^{[3
,4
,6
]}

Loeck, Steffen ^{[1
,2
,3
,4
,7
,8
]}

Krause, Mechthild ^{[1
,2
,3
,4
,5
,6
,7
,8
]}

Beyreuther, Elke ^{[3
,4
,9
]}

von Neubeck, Claere ^{[1
,2
,3
,4
,10
]}

机构：

[1] German Canc Consortium DKTK, Partner Site Dresden, D-69120 Heidelberg, Germany

[2] German Canc Res Ctr, D-69120 Heidelberg, Germany

[3] Tech Univ Dresden, Fac Med, OncoRay Natl Ctr Radiat Res Oncol, Helmholtz Zentrum Dresden Rossendorf, D-01309 Dresden, Germany

[4] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, Helmholtz Zentrum Dresden Rossendorf, D-01309 Dresden, Germany

[5] Helmholtz Zentrum Dresden Rossendorf, Inst Radiooncol OncoRay, D-01328 Dresden, Germany

[6] Natl Ctr Tumor Dis NCT, Partner Site Dresden, D-01307 Dresden, Germany

[7] Tech Univ Dresden, Fac Med, Dept Radiotherapy & Radiat Oncol, D-01309 Dresden, Germany

[8] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, D-01309 Dresden, Germany

[9] Helmholtz Zentrum Dresden Rossendorf, Inst Radiat Phys, D-01328 Dresden, Germany

[10] Univ Duisburg Essen, Univ Hosp Essen, Dept Particle Therapy, D-45147 Essen, Germany

来源：

CANCERS | 2020年 / 12卷 / 06期

基金：

欧盟地平线“2020”;

关键词：

tumor biology; thin-cut tissue slices; proton beam radiotherapy; head and neck cancer; organotypic brain slice culture; DNA damage; RESIDUAL GAMMA-H2AX FOCI; DNA-DAMAGE; ASSAY; IRRADIATION; RADIATION; CELLS; HEAD; RADIOSENSITIVITY; MICROGLIA; THERAPY;

D O I：

10.3390/cancers12061589

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A challenge in cancer research is the definition of reproducible, reliable, and practical models, which reflect the effects of complex treatment modalities and the heterogeneous response of patients. Proton beam radiotherapy (PBRT), relative to conventional photon-based radiotherapy, offers the potential for iso-effective tumor control, while protecting the normal tissue surrounding the tumor. However, the effects of PBRT on the tumor microenvironment and the interplay with newly developed chemo- and immunotherapeutic approaches are still open for investigation. This work evaluated thin-cut tumor slice cultures (TSC) of head and neck cancer and organotypic brain slice cultures (OBSC) of adult mice brain, regarding their relevance for translational radiooncology research. TSC and OBSC were treated with PBRT and investigated for cell survival with a lactate dehydrogenase (LDH) assay, DNA repair via the DNA double strand break marker gamma H2AX, as well as histology with regards to morphology. Adult OBSC failed to be an appropriate model for radiobiological research questions. However, histological analysis of TSC showed DNA damage and tumor morphological results, comparable to known in vivo and in vitro data, making them a promising model to study novel treatment approaches in patient-derived xenografts or primary tumor material.

引用

页码：1 / 19

页数：19

共 69 条

[31] Extreme hypofractionated proton radiotherapy for prostate cancer using pencil beam scanning: Dosimetry, acute toxicity and preliminary results [J].