Large-Scale Profiling of Archival Lymph Nodes Reveals Pervasive Remodeling of the Follicular Lymphoma Methylome

被引：36
作者：
Killian, J. Keith [1 ,2 ]
Bilke, Sven [1 ]
Davis, Sean [1 ]
Walker, Robert L. [1 ]
Killian, M. Scott [5 ]
Jaeger, Erich B. [4 ]
Chen, Yidong [1 ]
Hipp, Jason [2 ]
Pittaluga, Stefania [2 ]
Raffeld, Mark [2 ]
Cornelison, Robert [1 ]
Smith, William I., Jr. [3 ]
Bibikova, Marina [4 ]
Fan, Jian-Bing [4 ]
Emmert-Buck, Michael R. [2 ]
Jaffe, Elaine S. [2 ]
Meltzer, Paul S. [1 ]
机构：
[1] NIH, Genet Branch, NCI, Bethesda, MD 20892 USA
[2] NIH, Pathol Lab, NCI, Bethesda, MD 20892 USA
[3] Suburban Hosp, Dept Pathol, Bethesda, MD USA
[4] Illumina Inc, San Diego, CA USA
[5] Univ Calif San Francisco, Sch Med, San Francisco, CA USA
来源：
CANCER RESEARCH | 2009年 / 69卷 / 03期
关键词：
UNIVERSAL BEAD ARRAYS; B-CELL LYMPHOMA; DNA METHYLATION; GENE-EXPRESSION; CANCER;
D O I：
10.1158/0008-5472.CAN-08-2984
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Emerging technologies allow broad profiling of the cancer genome for differential DNA methylation relative to benign cells. Herein, bisulfite-modified DNA from lymph nodes with either reactive hyperplasia or follicular lymphoma (FL) were analyzed using a commercial C/UpG genotyping assay. Two hundred fifty-nine differentially methylated targets (DMT) distributed among 183 unique genes were identified in FL. Comparison of matched formalin-fixed, paraffin-embedded and frozen surgical pathology replicates showed the complete preservation of the cancer methylome among differently archived tissue specimens. Analysis of the DMT profile is consistent with a pervasive epigenomic remodeling process in FL that affects predominantly nonlymphoid genes. [Cancer Res 2009;69(3):758-64]
引用
收藏
页码：758 / 764
页数：7
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