Effect of delayed entry on performance of the BACT/ALERT FAN PLUS bottles in the BACT/ALERT VIRTUO blood culture system

被引：8

作者：

Adamik, Mary ^{[1
]}

Hutchins, Anne ^{[1
]}

Mangilit, Jasmin ^{[1
]}

Katzin, Betsy ^{[1
]}

Totty, Heather ^{[1
]}

Deol, Parampal ^{[1
]}

机构：

[1] BioMerieux Inc, Durham, NC 27712 USA

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2021年 / 40卷 / 04期

关键词：

Blood culture; BACT/ALERT (R); VIRTUO (R); Sepsis; Delayed entry; Pre-incubation; TRANSPORT TIME; SEVERE SEPSIS; DIAGNOSIS; THERAPY; MICROORGANISMS; MANAGEMENT; RECOVERY; 3D;

D O I：

10.1007/s10096-020-04042-z

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Delayed entry of patient blood culture samples into a microbial detection system is unavoidable at times, due to off-shift staffing or transporting samples to centralized laboratories. Pre-incubation time and temperature of blood culture bottles are the most critical factors impacting recovery and detection of microorganisms. A total of 1377 BACT/ALERT (R) (BTA) Fastidious Antimicrobial Neutralization (FAN (R) PLUS) bottles (FA PLUS, FN PLUS, and PF PLUS) were tested after delayed entry times of 24 and 36 h at 20-25 degrees C (room temperature, RT) prior to loading into the BACT/ALERT (R) VIRTUO (R) microbial detection system (VIRTUO). Clinically relevant organisms were inoculated into bottles with 5-84 colony forming units (CFU) per bottle, and human blood (0 to 10 mL), and then loaded into the VIRTUO. When bottles were loaded without delay, a mean time to detection (TTD) of 9.6 h was observed. For delayed bottles, the TTD reported by the VIRTUO was added to the 24-h and 36-h delay times and resulted in average time to results of 32.5 h and 42.5 h, respectively. The FAN PLUS bottles in conjunction with the VIRTUO produced acceptable results when delays up to 24 h at 20-25 degrees C occur in loading.

引用

页码：699 / 705

页数：7

共 32 条

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