Blood reperfusion of affected limbs is the most effective therapy for peripheral vascular thrombotic disease, restoring nutrition and blood flow to threatened tissues. Because it is more cost-effective than other thrombolytics, urokinase (UK) is widely used to treat venous thrombosis in China. However, its use is limited because of the risk of UK-related hemorrhagic complications. UK-coated nanoparticles (NPs) may decrease adverse effects while simultaneously increasing thrombolytic benefits. The aim of this study was to combine the sustained-release properties of NPs with the clinical benefits of catheter-directed thrombolysis (CDT) to create a promising new therapy. NPs were prepared via self-assembled chitosan and tripolyphosphate, introduced into a thrombosis model in New Zealand white rabbits, and the ratio of the residual thrombus cross-sectional area to the vascular cross-sectional area was calculated. The NPs had a drug-bearing efficiency of 14.5 +/- A 1.3 %, an encapsulation efficiency of 94.8 +/- A 2.1 % while the particle size of UK-coated NPs was 236 nm. Transmission electron microscopy results showed that the shape of the NPs were spherical and regular. Whether delivered by intravenation or catheter, UK-coated NPs produced a significant increase in the thrombolytic effect compared with free UK and confirmed the superiority of CDT for improving clot lysis over drug-induced systemic thrombolysis. The intravenous NPs caused an abnormal increase in fibrinogen. In conclusion, a water-soluble UK-WCS-NP suspension with good encapsulation efficiency was easily prepared UK-WCS-NPs were capable of maintaining UK activity, provided sustained-release of UK and exhibited better thrombolytic function than free UK.
机构:
Kims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Konyang Univ, Myong Gok Eye Res Inst, Seoul, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Baek, Seung-Hee
;
Park, Sung Jin
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机构:
Kims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Konyang Univ, Myong Gok Eye Res Inst, Seoul, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Park, Sung Jin
;
Jin, Su-Eon
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机构:
Seoul Natl Univ, Coll Pharm, Lab Excellency Drug & Gene Delivery, Songnam 463707, Kyungki Do, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Jin, Su-Eon
;
Kim, Jin-Ki
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机构:
Seoul Natl Univ, Coll Pharm, Lab Excellency Drug & Gene Delivery, Songnam 463707, Kyungki Do, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Kim, Jin-Ki
;
Kim, Chong-Kook
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机构:
Seoul Natl Univ, Coll Pharm, Lab Excellency Drug & Gene Delivery, Songnam 463707, Kyungki Do, South Korea
Inje Univ, Coll Engn, Dept Pharmaceut Engn, Gimhae, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Kim, Chong-Kook
;
Hwang, Jeong-Min
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机构:
Seoul Natl Univ, Dept Ophthalmol, Bundang Hosp, Coll Med, Songnam 463707, Kyungki Do, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
机构:
Nanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
E China Normal Univ, Inst Mol & Expt Therapeut, Shanghai 200062, Peoples R ChinaNanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
Bi, Feng
;
Zhang, Jing
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机构:
Nanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
E China Normal Univ, Inst Mol & Expt Therapeut, Shanghai 200062, Peoples R ChinaNanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
Zhang, Jing
;
Su, Yijing
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机构:
Nanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R ChinaNanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
Su, Yijing
;
Tang, Yan-Chun
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机构:
Nanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R ChinaNanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
Tang, Yan-Chun
;
Liu, Jian-Ning
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Nanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
E China Normal Univ, Inst Mol & Expt Therapeut, Shanghai 200062, Peoples R ChinaNanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
机构:
Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R ChinaUniv Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China
Chen, Meiwan
;
Zhong, Zhangfeng
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机构:
Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R ChinaUniv Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China
Zhong, Zhangfeng
;
Tan, Wen
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机构:
Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R ChinaUniv Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China
Tan, Wen
;
Wang, Shengpeng
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机构:
Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R ChinaUniv Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China
Wang, Shengpeng
;
Wang, Yitao
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h-index: 0
机构:
Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R ChinaUniv Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China
机构:
Kims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Konyang Univ, Myong Gok Eye Res Inst, Seoul, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Baek, Seung-Hee
;
Park, Sung Jin
论文数: 0引用数: 0
h-index: 0
机构:
Kims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Konyang Univ, Myong Gok Eye Res Inst, Seoul, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Park, Sung Jin
;
Jin, Su-Eon
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Coll Pharm, Lab Excellency Drug & Gene Delivery, Songnam 463707, Kyungki Do, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Jin, Su-Eon
;
Kim, Jin-Ki
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Coll Pharm, Lab Excellency Drug & Gene Delivery, Songnam 463707, Kyungki Do, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Kim, Jin-Ki
;
Kim, Chong-Kook
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Coll Pharm, Lab Excellency Drug & Gene Delivery, Songnam 463707, Kyungki Do, South Korea
Inje Univ, Coll Engn, Dept Pharmaceut Engn, Gimhae, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
Kim, Chong-Kook
;
Hwang, Jeong-Min
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Dept Ophthalmol, Bundang Hosp, Coll Med, Songnam 463707, Kyungki Do, South KoreaKims Eye Hosp, Dept Ophthalmol, Seoul, South Korea
机构:
Nanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
E China Normal Univ, Inst Mol & Expt Therapeut, Shanghai 200062, Peoples R ChinaNanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
Bi, Feng
;
Zhang, Jing
论文数: 0引用数: 0
h-index: 0
机构:
Nanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
E China Normal Univ, Inst Mol & Expt Therapeut, Shanghai 200062, Peoples R ChinaNanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
Zhang, Jing
;
Su, Yijing
论文数: 0引用数: 0
h-index: 0
机构:
Nanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R ChinaNanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
Su, Yijing
;
Tang, Yan-Chun
论文数: 0引用数: 0
h-index: 0
机构:
Nanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R ChinaNanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
Tang, Yan-Chun
;
Liu, Jian-Ning
论文数: 0引用数: 0
h-index: 0
机构:
Nanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
E China Normal Univ, Inst Mol & Expt Therapeut, Shanghai 200062, Peoples R ChinaNanjing Univ, Inst Mol Med, Nanjing 210093, Peoples R China
机构:
Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R ChinaUniv Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China
Chen, Meiwan
;
Zhong, Zhangfeng
论文数: 0引用数: 0
h-index: 0
机构:
Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R ChinaUniv Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China
Zhong, Zhangfeng
;
Tan, Wen
论文数: 0引用数: 0
h-index: 0
机构:
Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R ChinaUniv Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China
Tan, Wen
;
Wang, Shengpeng
论文数: 0引用数: 0
h-index: 0
机构:
Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R ChinaUniv Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China
Wang, Shengpeng
;
Wang, Yitao
论文数: 0引用数: 0
h-index: 0
机构:
Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R ChinaUniv Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China