Establishment of a Salmonella tester strain highly sensitive to mutagenic heterocyclic amines

被引：5

作者：

Kamataki, T ^{[1
]}

Suzuki, A

Kushida, H

Iwata, H

Watanabe, M

Nohmi, T

Fujita, K

机构：

[1] Hokkaido Univ, Grad Sch Pharmaceut Sci, Div Pharmacobiodynam, Lab Drug Metab,Kita Ku, Sapporo, Hokkaido 0600812, Japan

[2] Natl Inst Canc Res, Div Biochem, Chuo Ku, Tokyo 1040045, Japan

[3] Natl Inst Hlth Sci, Div Genet & Mutagenesis, Setagaya Ku, Tokyo 1580098, Japan

来源：

CANCER LETTERS | 1999年 / 143卷 / 02期

关键词：

Salmonella tester strain; mutagenic heterocyclic amines;

D O I：

10.1016/S0304-3835(99)00138-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A co-expression plasmid (p1A2OR) carrying human CYP1A2 and NADPH-P450 reductase cDNAs and an expression plasmid (pOAT) carrying Salmonella TA1538/ARO cells. The CYP and OAT expressed in the Salmonella cells showed catalytic activity. The TA1538/ARO strain exhibited high sensitivity to heterocyclic amines (HCAs) such as 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,8-dimenthylimidazo[4,5-f]quinoline (MeIQx), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) as compared with the parent Ames tester strain TA1538. It is suggested that the intracellular expression of drug-metabolising enzymes makes the Salmonella cells highly sensitive to the HCAs. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：113 / 116

页数：4