Design of a prototype flow microreactor for synthetic biology in vitro

被引：27

作者：

Boehm, Christian R. ^{[1
,2
,3
]}

Freemont, Paul S. ^{[2
,3
]}

Ces, Oscar ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Inst Chem Biol, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Ctr Synthet Biol & Innovat, London SW7 2AZ, England

[3] Univ London Imperial Coll Sci Technol & Med, Div Mol Biosci, London SW7 2AZ, England

来源：

LAB ON A CHIP | 2013年 / 13卷 / 17期

基金：

英国工程与自然科学研究理事会;

关键词：

MICROFLUIDIC CHANNELS; KINETICS; ENZYMES; SYSTEMS; LACTOSE;

D O I：

10.1039/c3lc50231g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

As a reference platform for in vitro synthetic biology, we have developed a prototype flow microreactor for enzymatic biosynthesis. We report the design, implementation, and computer-aided optimisation of a three-step model pathway within a microfluidic reactor. A packed bed format was shown to be optimal for enzyme compartmentalisation after experimental evaluation of several approaches. The specific substrate conversion efficiency could significantly be improved by an optimised parameter set obtained by computational modelling. Our microreactor design provides a platform to explore new in vitro synthetic biology solutions for industrial biosynthesis.

引用

页码：3426 / 3432

页数：7

共 34 条

[1] Synthetic biology: new engineering rules for an emerging discipline [J].

Andrianantoandro, Ernesto ;

Basu, Subhayu ;

Karig, David K. ;

Weiss, Ron .

MOLECULAR SYSTEMS BIOLOGY, 2006, 2 (1) :2006.0028

[2] Setting the standard in synthetic biology [J].

Arkin, Adam .

NATURE BIOTECHNOLOGY, 2008, 26 (07) :771-774

[3]

Baek TJ, 2007, J MICROBIOL BIOTECHN, V17, P1826

[4] Physics and applications of microfluidics in biology [J].

Beebe, DJ ;

Mensing, GA ;

Walker, GM .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2002, 4 :261-286

[5]

Deen W. M., ANAL TRANSPORT PHENO, P22

[6]

Ehrfeld W., 2000, Microreactors: new technology for modern chemistry, DOI DOI 10.1002/3527601953

[7] Foundations for engineering biology [J].

Endy, D .

NATURE, 2005, 438 (7067) :449-453

[8] Cellulases: Agents for fiber modification or bioconversion? The effect of substrate accessibility on cellulose enzymatic hydrolyzability [J].

Esteghlalian, AR ;

Mansfield, SD ;

Saddler, JN .

BIOTECHNOLOGY IN THE PULP AND PAPER INDUSTRY: 8TH ICBPPI, 2002, 21 :21-36

[9] Sequential Immobilization of Enzymes in Microfluidic Channels for Cascade Reactions [J].

Fornera, Sara ;

Kuhn, Phillip ;

Lombardi, Dario ;

Schlueter, A. Dieter ;

Dittrich, Petra S. ;

Walde, Peter .

CHEMPLUSCHEM, 2012, 77 (02) :98-101

[10] Synthetic biology projects in vitro [J].

Forster, Anthony C. ;

Church, George M. .

GENOME RESEARCH, 2007, 17 (01) :1-6

← 1 2 3 4 →