Simulation of two dimensional electrophoresis and tandem mass spectrometry for teaching proteomics

被引：13

作者：

Fisher, Amanda ^{[1
]}

Sekera, Emily ^{[1
]}

Payne, Jill ^{[2
]}

Craig, Paul ^{[1
]}

机构：

[1] Rochester Inst Technol, Sch Chem & Mat Sci, Rochester, NY 14623 USA

[2] Rochester Inst Technol, Dept Comp Sci, Rochester, NY 14623 USA

来源：

BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION | 2012年 / 40卷 / 06期

关键词：

Biotechnology education; computational biology; computers in research and teaching; genomics proteomics bioinformatics; laboratory exercises; using simulation and internet resources for teaching; IDENTIFICATION; CHEMISTRY; EXERCISE;

D O I：

10.1002/bmb.20651

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In proteomics, complex mixtures of proteins are separated (usually by chromatography or electrophoresis) and identified by mass spectrometry. We have created 2DE Tandem MS, a computer program designed for use in the biochemistry, proteomics, or bioinformatics classroom. It contains two simulations-2D electrophoresis and tandem mass spectrometry. The two simulations are integrated together and are designed to teach the concept of proteome analysis of prokaryotic and eukaryotic organisms. 2DE-Tandem MS can be used as a freestanding simulation, or in conjunction with a wet lab, to introduce proteomics in the undergraduate classroom. 2DE Tandem MS is a free program available on Sourceforge at https://sourceforge.net/projects/jbf/. It was developed using Java Swing and functions in Mac OSX, Windows, and Linux, ensuring that every student sees a consistent and informative graphical user interface no matter the computer platform they choose. Java must be installed on the host computer to run 2DE Tandem MS. Example classroom exercises are provided in the Supporting Information. (c) 2012 by The International Union of Biochemistry and Molecular Biology

引用

页码：393 / 399

页数：7

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