Ultrafast fluorescence spectroscopy of photoactive yellow protein

被引:16
|
作者
Hanada, H [1 ]
Kanematsu, Y
Kinoshita, S
Kumauchi, M
Sasaki, J
Tokunaga, F
机构
[1] Osaka Univ, Grad Sch Sci, Toyonaka, Osaka 5600043, Japan
[2] Osaka Univ, Venture Business Lab, Suita, Osaka 5650871, Japan
[3] Unisoku Co Ltd, Hirakata, Osaka 5730131, Japan
关键词
photoactive yellow protein; optical Kerr shutter; time-resolved fluorescence;
D O I
10.1016/S0022-2313(01)00329-5
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Using the optical Kerr shutter method, room temperature time-resolved fluorescence spectroscopy of photoactive yellow protein (PYP) has been performed. Spectral profiles of the time dependent fluorescence have been measured as snapshots with high time resolution of about 300 fs. For the first time, spectral narrowing in the ps-time region was directly observed, which is consistent with the previously reported results of the faster decay on both sides of the fluorescence peak. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:593 / 596
页数:4
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