Characterization of two new high-grade B-cell lymphoma cell lines with MYC and BCL2 rearrangements that are suitable for in vitro drug sensitivity studies

被引：1

作者：

Dheur, Marie-Sophie ^{[1
]}

Poirel, Helene A. ^{[2
]}

Ameye, Genevieve ^{[2
]}

Tilman, Gaelle ^{[2
]}

Saussoy, Pascale ^{[3
]}

Defour, Jean-Philippe ^{[3
]}

Camboni, Alessandra ^{[4
,5
]}

Van den Neste, Eric ^{[5
]}

Coulie, Pierre G. ^{[1
]}

van Baren, Nicolas ^{[1
]}

机构：

[1] Catholic Univ Louvain, Duve Inst, Cellular Genet Unit, Ave Hippocrate 75-B1-74-04, B-1200 Brussels, Belgium

[2] Catholic Univ Louvain, Clin Univ St Luc, Ctr Genet Humaine, Brussels, Belgium

[3] Catholic Univ Louvain, Clin Univ St Luc, Dept Clin Biol, Brussels, Belgium

[4] Catholic Univ Louvain, Clin Univ St Luc, Dept Pathol, Brussels, Belgium

[5] Catholic Univ Louvain, Clin Univ St Luc, Dept Hematol, Brussels, Belgium

来源：

LEUKEMIA & LYMPHOMA | 2019年 / 60卷 / 04期

关键词：

High-grade double-hit lymphoma; cell lines; MYC; BCL2; RITUXIMAB PLUS CYCLOPHOSPHAMIDE; FOLLICULAR LYMPHOMA; MUTATIONS; VINCRISTINE; DOXORUBICIN; EXPRESSION; INHIBITOR; PROTEINS; ABT-199;

D O I：

10.1080/10428194.2018.1508663

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

High-grade B-cell lymphomas with MYC and BCL2 or BCL6 rearrangements are highly aggressive B-cell lymphomas called double-hit lymphomas (HGBL-DH). They are particularly refractory to standard treatments and carry a poor prognosis. Fragments of resected tumoral lymph nodes from two HGBL-DH patients were put in culture. Continuously proliferating cells were characterized and compared with the original tumors. In both cases, the proliferating cells and the tumor displayed MYC and BCL2 rearrangements. Both cell lines (called LB5848-LYMP and LB5871-LYMP) presented a high proliferation rate and were maintained in culture for more than one year. Upon injection in immunodeficient mice, LB5848-LYMP gave rise to lymphoid tumors. In vitro treatment of these cell lines with a BCL2-inhibitory drug (ABT-199) selectively stopped their proliferation. These new cell lines represent valuable tools for studying HGBL-DH and for the in vitro testing of candidate therapies targeting HGBL-DH. LB5848-LYMP is also suitable for similar experiments in vivo.

引用

页码：1043 / 1052

页数：10

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