mRNA stability and localisation of the low-temperature-responsive barley gene family blt14

被引：39

作者：

Phillips, JR ^{[1
]}

Dunn, MA ^{[1
]}

Hughes, MA ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE, DEPT BIOCHEM & GENET, NEWCASTLE UPON TYNE NE4 2HH, TYNE & WEAR, ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1997年 / 33卷 / 06期

关键词：

barley; cold acclimation; multigene family; mRNA stability; organ specific expression; post-transcriptional regulation;

D O I：

10.1023/A:1005717613224

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcription and translation inhibitors have been used to investigate the role of mRNA stability in the low-temperature-regulated expression of the post-transcriptionally controlled low temperature responsive barley gene family, blt14. Genomic clones (blt14.1, blt14.2) representing additional members of the blt14 gene family have been isolated and sequenced. Gene specific probes have been used to analyse the spatial expression of each individual member of the blt14 gene family. Findings indicate that all of the genes an responsive to low temperature, but the organ distribution is different for each gene. The results indicate that blt14.0 mRNA is stabilised by a low-temperature-dependent protein factor. Taken together, the results suggest that organ-specific post-transcriptional mechanisms are important in the low-temperature regulation of blt14 gene expression.

引用

页码：1013 / 1023

页数：11

共 41 条

[1] DIFFERENTIAL ORGAN-SPECIFIC EXPRESSION OF 3 POLY(A)-BINDING-PROTEIN GENES FROM ARABIDOPSIS-THALIANA [J].