Electroporation as a tool to transfer the plasmid pRL489 in Oscillatoria MKU 277

被引：11

作者：

Ravindran, C. R. Marutha

Suguna, S.

Shanmugasundaram, S.

机构：

[1] Univ Texas, Hlth Sci Ctr, Dept Pharmacol, San Antonio, TX 78229 USA

[2] Madurai Kamaraj Univ, Sch Biol Sci, Dept Microbial Technol, DBT Ctr BGA Biofetilizer, Madurai 625021, Tamil Nadu, India

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 66卷 / 01期

关键词：

cyanobacteria; biofertilizer; Oscillatoria; electroporation; periplasmic nuclease; kanamycin; CYANOBACTERIUM; TRANSFORMATION; TRANSPOSON; COMPLEX;

D O I：

10.1016/j.mimet.2005.11.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To establish the gene transfer system in the cyanobacteria, we successfully introduced the shuttle plasmid pRL489 into Oscillatoria MKU 277 by electroporation with transfort-nation frequencies of up to 102 cfu/mu g of plasmid DNA. These findings suggest that Oscillatoria MKU 277 can be used as an experimental tool for genetic maneuvering. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：174 / 176

页数：3

共 9 条

[1] Isolation and characterization of a chlorate-resistant mutant (Clo-R) of the symbiotic cyanobacterium Nostoc ANTH:: Heterocyst formation and N2-fixation in the presence of nitrate, and evidence for separate nitrate and nitrite transport systems [J].