SPIM-fluid: open source light-sheet based platform for high-throughput imaging

被引：62

作者：

Gualda, Emilio J. ^{[1
]}

Pereira, Hugo ^{[1
,2
,3
]}

Vale, Tiago ^{[1
]}

Estrada, Marta Falcao ^{[2
,3
]}

Brito, Catarina ^{[2
,3
]}

Moreno, Nuno ^{[1
]}

机构：

[1] Inst Gulbenkian Ciencias, Advance Imaging Lab, P-2780156 Oeiras, Portugal

[2] IBET Inst Biol Expt & Tecnol, P-2781901 Oeiras, Portugal

[3] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal

来源：

BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 11期

关键词：

MICROSCOPY;

D O I：

10.1364/BOE.6.004447

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening. (C) 2015 Optical Society of America

引用

页码：4447 / 4456

页数：10

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