Microscopic and Molecular Detection of Cryptosporidium andersoni and Cryptosporidium xiaoi in Wastewater Samples of Tehran Province, Iran

被引:0
作者
Hatam-Nahavandi, Kareem [1 ,2 ,3 ]
Mohebali, Mehdi [2 ,4 ]
Mahvi, Amir-Hossein [5 ,6 ]
Keshavarz, Hossein [2 ,4 ]
Najafian, Hamid-Reza [2 ]
Mirjalali, Hamed [7 ,8 ]
Rezaei, Sasan [2 ]
Rezaeian, Mostafa [2 ,4 ]
机构
[1] Zahedan Univ Med Sci, Sch Med, Dept Parasitol & Mycol, Zahedan, Iran
[2] Univ Tehran Med Sci, Sch Publ Hlth, Dept Parasitol & Mycol, Tehran, Iran
[3] Zahedan Univ Med Sci, Infect Dis & Trop Med Res Ctr, Zahedan, Iran
[4] Univ Tehran Med Sci, CREPI, Tehran, Iran
[5] Sch Publ Hlth, Dept Environm Hlth Engn, Tehran, Iran
[6] Univ Tehran Med Sci, IER, Ctr Water Qual Res, Tehran, Iran
[7] Shahid Beheshti Univ Med Sci, Res Inst Gastroenterol & Liver Dis, Foodborne & Waterborne Dis Res Ctr, Tehran, Iran
[8] Shahid Beheshti Univ Med Sci, Res Inst Gastroenterol & Liver Dis, Gastroenterol & Liver Dis Res Ctr, Tehran, Iran
关键词
Cryptosporidium andersoni; Cryptosporidium xiaoi; Iran; Wastewater; TREATMENT PLANTS; SURFACE-WATER; REMOVAL EFFICIENCY; GIARDIA; SLAUGHTERHOUSE; INFECTION; GENOTYPES; HUMANS; URBAN;
D O I
暂无
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Background: As a waterborne pathogen, Cryptosporidium is one of the most common causes of gastroenteritis in human and hoofed livestock animals. This study aimed to investigate the distribution of Cryptosporidium spp. in human and livestock wastewaters in Iran, by the 18S rRNA sequence analysis. Methods: A total of 54 raw wastewater samples collected from three urban treatment plants and two slaughterhouses during 2014-2015 in Tehran, Iran. The presence of the Cryptosporidium oocysts was assessed by immunofluorescence with monoclonal antibodies. To characterize the oocysts at the molecular level, the 18S rRNA gene of Cryptosporidium was PCR amplified and sequenced. Results: Of the 54 wastewater samples examined, 34 (62.9%) were positive for Cryptosporidium oocysts using the IFA. Of these, 70.5% (24/34) were positive by PCR, that 91.6% (22/24) were successfully sequenced. The species of C. andersoni (95.4%) and C. xiaoi (4.6%) were detected in livestock wastewater samples. Conclusion: C. andersoni was the major Cryptosporidium sp. found in the aquatic environmental wastewater samples. The high rate of detection of C. andersoni in domestic wastewater was probably the result of the predominancy of this species in cattle herds in Iran. The current study is the first report of C. xiaoi in Iran.
引用
收藏
页码:499 / 506
页数:8
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