Quencher as leaving group: Efficient detection of DNA-joining reactions

被引：110

作者：

Sando, S ^{[1
]}

Kool, ET ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 10期

关键词：

D O I：

10.1021/ja017328s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe a new fluorescence reporting strategy in which dabsyl, a well-known quencher, activates a hydroxyl group in a probe to convert it to a leaving group. When a nucleophilic phosphorothioate probe binds adjacent to a dabsyl quenched probe, autoligation occurs, releasing the quencher, and lighting up the probes, This signal change can be used to detect single nucleotide differences in DNA without enzymes or reagents. Copyright © 2002 American Chemical Society.

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页码：2096 / 2097

页数：2

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