Boosting Salt Resistance of Short Antimicrobial Peptides

被引：101

作者：

Chu, Hung-Lun ^{[1
,2
]}

Yu, Hui-Yuan ^{[1
,2
]}

Yip, Bak-Sau ^{[1
,2
,3
]}

Chih, Ya-Han ^{[1
,2
]}

Liang, Chong-Wen ^{[1
,2
]}

Cheng, Hsi-Tsung ^{[1
,2
]}

Cheng, Jya-Wei ^{[1
,2
]}

机构：

[1] Natl Tsing Hua Univ, Inst Biotechnol, Hsinchu, Taiwan

[2] Natl Tsing Hua Univ, Dept Med Sci, Hsinchu, Taiwan

[3] Natl Taiwan Univ Hosp, Hsinchu Branch, Dept Neurol, Hsinchu, Taiwan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2013年 / 57卷 / 08期

关键词：

ANTIBACTERIAL; HYDROPHOBICITY; DESIGN;

D O I：

10.1128/AAC.00252-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The efficacies of many antimicrobial peptides are greatly reduced under high salt concentrations, therefore limiting their use as pharmaceutical agents. Here, we describe a strategy to boost salt resistance and serum stability of short antimicrobial peptides by adding the nonnatural bulky amino acid beta-naphthylalanine to their termini. The activities of the short salt-sensitive tryptophan-rich peptide S1 were diminished at high salt concentrations, whereas the activities of its beta-naphthylalanine end-tagged variants were less affected.

引用

页码：4050 / 4052

页数：3

共 25 条

[1] Ultrashort Peptide Bioconjugates Are Exclusively Antifungal Agents and Synergize with Cyclodextrin and Amphotericin B [J].

Arnusch, Christopher J. ;

Ulm, Hannah ;

Josten, Michaele ;

Shadkchan, Yana ;

Osherov, Nir ;

Sahl, Hans-Georg ;

Shai, Yechiel .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2012, 56 (01) :1-9

[2] Conjugation of a magainin analogue with lipophilic acids controls hydrophobicity, solution assembly, and cell selectivity [J].

Avrahami, D ;

Shai, Y .

BIOCHEMISTRY, 2002, 41 (07) :2254-2263

[3] De nova generation of cationic antimicrobial peptides: Influence of length and tryptophan substitution on antimicrobial activity [J].

Deslouches, B ;

Phadke, SM ;

Lazarevic, V ;

Cascio, M ;

Islam, K ;

Montelaro, RC ;

Mietzner, TA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (01) :316-322

[4] Cluster analysis and display of genome-wide expression patterns [J].

Eisen, MB ;

Spellman, PT ;

Brown, PO ;

Botstein, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14863-14868

[5] Salt-resistant alpha-helical cationic antimicrobial peptides [J].

Friedrich, C ;

Scott, MG ;

Karunaratne, N ;

Yan, H ;

Hancock, REW .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (07) :1542-1548

[6] Human beta-defensin-1 is a salt-sensitive antibiotic in lung that is inactivated in cystic fibrosis [J].

Goldman, MJ ;

Anderson, GM ;

Stolzenberg, ED ;

Kari, UP ;

Zasloff, M ;

Wilson, JM .

CELL, 1997, 88 (04) :553-560

[7] Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies [J].

Hancock, Robert E. W. ;

Sahl, Hans-Georg .

NATURE BIOTECHNOLOGY, 2006, 24 (12) :1551-1557

[8] Intramolecular disulfide bonds enhance the antimicrobial and lytic activities of protegrins at physiological sodium chloride concentrations [J].

Harwig, SSL ;

Waring, A ;

Yang, HJ ;

Cho, Y ;

Tan, L ;

Lehrer, RI .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 240 (02) :352-357

[9] Effects of pH and salinity on the antimicrobial properties of clavanins [J].

Lee, IH ;

Cho, Y ;

Lehrer, RI .

INFECTION AND IMMUNITY, 1997, 65 (07) :2898-2903

[10] Ultrashort antibacterial and antifungal lipopeptides [J].

Makovitzki, Arik ;

Avrahami, Dorit ;

Shai, Yechiel .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (43) :15997-16002

← 1 2 3 →