Polyadenylation is dispensable for encapsidation and reverse transcription of hepatitis B viral pregenomic RNA

被引:0
作者
Lee, Hye-Jin [1 ]
Lee, Jehan [1 ]
Shin, Myeong-Kyun [1 ]
Ryu, Wang-Shick [1 ]
机构
[1] Yonsei Univ, Dept Biochem, Seoul 120749, South Korea
关键词
encapsidation; hepatitis B virus; polyadenylation; reverse transcription;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A hepadnaviruses replicates its DNA genome via reverse transcription of an RNA template (pregenomic RNA or pgRNA), which has a cap structure at the 5' end and a poly(A) tail at the 3' end. We have previously shown that the 5' cap is indispensable for encapsidation of the pgRNA. A speculative extension of the above finding is that the cap contributes to encapsidation via its interaction with the poly(A) tail, possibly involving eIF4E-eIF4G-PABP interaction. To test this hypothesis, poly(A)-less pgRNAs were generated via cleavage by a cis-acting hepatitis delta virus ribozyme sequence. We found that accumulation of the poly(A)less pgRNA was markedly diminished, mostly likely due to its reduced stability. Importantly, however, the remaining poly(A)-less pgRNAs were nonetheless encapsidated and reverse transcribed normally when the reduced stability was taken account. Our finding clearly contradicts the notion that the poly(A) tail has any function in encapsidation and viral reverse transcription.
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页码:545 / 552
页数:8
相关论文
共 26 条
[1]   Base pairing between the 5′ half of ε and a cis-acting sequence, Φ, makes a contribution to the synthesis of minus-strand DNA for human hepatitis B virus [J].
Abraham, TM ;
Loeb, DD .
JOURNAL OF VIROLOGY, 2006, 80 (09) :4380-4387
[2]   Identification and characterization of mutations in hepatitis B virus resistant to lamivudine [J].
Allen, MI ;
Deslauriers, M ;
Andrews, CW ;
Tipples, GA ;
Walters, KA ;
Tyrrell, DLJ ;
Brown, N ;
Condreay, LD .
HEPATOLOGY, 1998, 27 (06) :1670-1677
[3]   HEPADNAVIRAL ASSEMBLY IS INITIATED BY POLYMERASE BINDING TO THE ENCAPSIDATION SIGNAL IN THE VIRAL-RNA GENOME [J].
BARTENSCHLAGER, R ;
SCHALLER, H .
EMBO JOURNAL, 1992, 11 (09) :3413-3420
[4]   NUCLEOTIDE-SEQUENCE OF THE HEPATITIS-B VIRUS GENOME (SUBTYPE AYW) CLONED IN ESCHERICHIA-COLI [J].
GALIBERT, F ;
MANDART, E ;
FITOUSSI, F ;
TIOLLAIS, P ;
CHARNAY, P .
NATURE, 1979, 281 (5733) :646-650
[5]  
Ganem D., 2001, FIELDS VIROLOGY, V2, P2923
[6]   Molecular mechanisms of translational control [J].
Gebauer, F ;
Hentze, MW .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2004, 5 (10) :827-835
[7]   cis-acting sequences in addition to donor and acceptor sites are required for template switching during synthesis of plus-strand DNA for duck hepatitis B virus [J].
Havert, MB ;
Loeb, DD .
JOURNAL OF VIROLOGY, 1997, 71 (07) :5336-5344
[8]   Evidence that the 5′-end cap structure is essential for encapsidation of hepatitis B virus pregenomic RNA [J].
Jeong, JK ;
Yoon, GS ;
Ryu, WS .
JOURNAL OF VIROLOGY, 2000, 74 (12) :5502-5508
[9]   A SHORT CIS-ACTING SEQUENCE IS REQUIRED FOR HEPATITIS-B VIRUS PREGENOME ENCAPSIDATION AND SUFFICIENT FOR PACKAGING OF FOREIGN RNA [J].
JUNKERNIEPMANN, M ;
BARTENSCHLAGER, R ;
SCHALLER, H .
EMBO JOURNAL, 1990, 9 (10) :3389-3396
[10]   Versatile PCR-mediated insertion or deletion mutagenesis [J].
Lee, J ;
Lee, HJ ;
Shin, MK ;
Ryu, WS .
BIOTECHNIQUES, 2004, 36 (03) :398-+