Lower Selenoprotein T Expression and Immune Response in the Immune Organs of Broilers with Exudative Diathesis Due to Selenium Deficiency

被引:36
作者
Pan, Tingru [1 ]
Liu, Tianqi [1 ]
Tan, Siran [1 ]
Wan, Na [1 ]
Zhang, Yiming [1 ]
Li, Shu [1 ]
机构
[1] Northeast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Selenium; Selenoprotein T; Exudative diathesis; Broiler; Immune organs; MESSENGER-RNA EXPRESSION; OXIDATIVE STRESS; DIETARY SELENIUM; GENE-EXPRESSION; CHICKEN; MECHANISMS; APOPTOSIS; CYTOKINES; SPLEEN; CELLS;
D O I
10.1007/s12011-017-1110-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The objective of the present study was to investigate whether dietary selenium (Se) deficiency would affect the expression of selenoprotein T (SelT) and immune response in the immune organs of broilers. Changes in expression of inflammatory cytokines and oxidative stress response caused by Se deficiency can lead to organism damage, which in turn leads to immune response. Sixty (1-day-old) broilers were divided into the control group and Se-deficiency group. Animal models with exudative diathesis were duplicated in the broilers by feeding them Se-deficient diet for 20 days. After the Se-deficient group exhibited symptoms of exudative diathesis, all the broilers were euthanized, and their immune organs were taken for analysis. The tissues including spleen, bursa of Fabricius, and thymus were treated to determine the pathological changes (including microscopic and ultramicroscopic), the messenger RNA (mRNA) expression levels of SelT and its synthetase (SecS and SPS1), cytokine mRNA expression levels, and antioxidant status. The microscopic and ultramicroscopic analyses showed that immune tissues were obviously injured in the Se-deficient group. The mRNA expression of SelT was decreased compared with that in the control group. Meanwhile, the mRNA expression levels of SecS and SPS1 were downregulated. In the Se-deficient group, the mRNA expression levels of IL-1R and IL-1 beta were higher than those of three control organs. Additionally, the IL-2 and INF-gamma mRNA expression levels were lower than those of the control group. The activity of CAT was decreased, and the contents of H2O2 and aEuro cent OH were increased due to Se deficiency. Pearson method analysis showed that the expression of SelT had a positive correlation with IL-2, INF-gamma, SecS, and SPS1 and a negative correlation with IL-1R and IL-1 beta. In summary, these data indicated that Se-deficient diet decreased the SelT expression and its regulation of oxidative stress, and it inhibited a pleiotropic mechanism of the immune response.
引用
收藏
页码:364 / 372
页数:9
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