Activity of extracellular compounds of Pseudomonas sp against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus

被引：16
作者：
Lopes, Lucilene P. [1 ]
Oliveira, Admiltom G., Jr. [1 ]
Beranger, Jamile P. O. [1 ]
Gois, Cintia G. [1 ]
Vasconcellos, Fernanda C. S. [1 ]
San Martin, Juca A. B. [2 ]
Andrade, Celia G. T. J. [2 ]
Mello, Joao C. P. [3 ]
Andrade, Galdino [1 ]
机构：
[1] Univ Estadual Londrina, Dept Microbiol, BR-86051990 Londrina, PR, Brazil
[2] Univ Estadual Londrina, Dept Biol Geral, BR-86051990 Londrina, PR, Brazil
[3] Univ Estadual Maringa, Dept Farm & Farmacol, BR-87020900 Maringa, Parana, Brazil
来源：
TROPICAL PLANT PATHOLOGY | 2012年 / 37卷 / 04期
关键词：
Antibiotic activity; antimicrobial; biological control; BIOLOGICAL-CONTROL; RHIZOBACTERIA; BIOCONTROL; SPECTRUM;
D O I：
10.1590/S1982-56762012000400001
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Bacterial leaf blight caused by Xanthomonas axonopodis is a major problem in the production of eucalyptus seedlings. The pathogen causes leaf lesions that reduce the photosynthetic area and limit plant growth. The objective of this study was to evaluate the antibiotic activity of secondary metabolic compounds of a Pseudomonas sp. against X. axonopodis, following extraction with dichloromethane and fractionation by vacuum liquid chromatography. The antibiotic activity of an ethyl acetate phase (F3 fraction) was determined by agar diffusion tests and bioautography. The minimum inhibitory concentration of F3 fraction and the action of F3 on the cell morphology of X. axonopodis was determined by scanning electron microscopy. Electron microscopy revealed changes in exopolysaccharides and morphology of treated cells. When eucalyptus seedlings were sprayed with the F3 fraction before or after inoculation with X. axonopodis in a greenhouse experiment the number of leaf lesions was respectively 93.9% and 89.7% lower than in untreated control plants. High antibiotic activity of the F3 fraction against X. axonopodis and leaf blight suggests that it has potential to control the disease in eucalyptus seedlings.
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收藏
页码：233 / 238
页数：6
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