Scale-up of two-step acid-catalysed glycerol pretreatment for production of oleaginous yeast biomass from sugarcane bagasse by Rhodosporidium toruloides

被引：28
作者：
Hassanpour, Morteza [1 ,2 ]
Abbasabadi, Mahsa [1 ,3 ]
Strong, James [1 ,3 ]
Gebbie, Leigh [1 ,3 ]
Te'o, Valentino Setoa Junior [1 ,3 ]
O'Hara, Ian M. [1 ,2 ]
Zhang, Zhanying [1 ,2 ]
机构：
[1] Queensland Univ Technol, Ctr Agr & Bioecon, Inst Future Environm, 2 George St, Brisbane, Qld 4000, Australia
[2] Queensland Univ Technol, Sci & Engn Fac, Sch Mech Med & Proc Engn, 2 George St, Brisbane, Qld 4000, Australia
[3] Queensland Univ Technol, Sci & Engn Fac, Sch Biol & Environm Sci, 2 George 5t, Brisbane, Qld 4000, Australia
来源：
BIORESOURCE TECHNOLOGY | 2020年 / 313卷
关键词：
Glycerol; Pretreatment; Rhodosporidium toruloides; Lipid; Carotenoid; MICROBIAL LIPID PRODUCTION; ORGANOSOLV PRETREATMENT; LIGNOCELLULOSIC BIOMASS; CO-UTILIZATION; FATTY-ACIDS; RICE STRAW; HYDROLYSATE; OIL; MIXTURE; LIGNIN;
D O I：
10.1016/j.biortech.2020.123666
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Two-step dilute acid and acid-catalysed glycerol pretreatment was developed to maximise sugar yield from sugarcane bagasse. At the laboratory scale, dilute acid pretreatment at 130 degrees C followed by acid-catalysed glycerol pretreatment at 170 degrees C led to a total sugar (C5 + C6) yield of 82%, 31% higher than that from one-step acid-catalysed glycerol pretreatment. At the pilot scale, the two-step dilute acid and acid-catalysed glycerol pretreatment led to a maximum sugar yield of 74%, 13% higher than that from one-step pretreatment with 52% reduction in glycerol usage. The enzymatic hydrolysate containing glucose and residual glycerol were used to produce microbial oils by a Rhodosporidium toruloides strain. A fed-batch cultivation strategy led to the pro-duction of 44.8 g/L cell mass, including 26.6 g/L oil, 8.6 g/L protein and 12.7 mg/L carotenoid. The cell mass and oil yields were 19% higher than those from batch cultivation as feedstock inhibition and catabolite re-pression were alleviated.
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页数：10
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