Acetylcholinesterase inhibition-based biosensor for amperometric detection of Sarin using single-walled carbon nanotube-modified ferrule graphite electrode

被引：34

作者：

Sharma, S. P. ^{[1
]}

Tomar, L. N. S. ^{[1
]}

Acharya, J. ^{[1
]}

Chaturvedi, A. ^{[1
]}

Suryanarayan, M. V. S. ^{[1
]}

Jain, Rajeev ^{[2
]}

机构：

[1] Def Res & Dev Estab, Synthet Chem Div, Gwalior 474002, MP, India

[2] Jiwaji Univ, Sch Studies Chem, Gwalior 474002, India

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2012年 / 166卷

关键词：

Acetylcholinesterase; Carboxylated single-walled carbon nanotube; Ferrule graphite electrode; Sarin; DFP; IMMOBILIZED ORGANOPHOSPHORUS HYDROLASE; COBALT PHTHALOCYANINE; ELECTROCHEMICAL BIOSENSORS; PASTE ELECTRODES; NERVE AGENTS; PESTICIDES; SENSOR; INSECTICIDE; RESIDUES; PARAOXON;

D O I：

10.1016/j.snb.2012.03.022

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, highly sensitive acetylcholinesterase (AChE) inhibition-based amperometric biosensor has been developed. AChE was immobilized by cross-linking with glutaraldehyde onto ferrule graphite electrode (FGE) modified with carboxylated single-walled carbon nanotube (cSWCNT). The properties of optimized biosensors are characterized by scanning electron microscopy (SEM), cyclic voltammograms (CVs) and electrochemical impedance spectroscopy (EIS). The synergistic action of cSWCNT/FGE showed excellent electrocatalytic activity with low applied potential for the detection of acetylthiocholine (ACTh). Sarin and diisopropylfluorophosphate (DFP) could be detected up to 25-35 nM and 15-20 nM respectively at 30-40% inhibition level of AChE and followed linearity in wide range concentration. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：616 / 623

页数：8

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