Acetylcholinesterase inhibition-based biosensor for amperometric detection of Sarin using single-walled carbon nanotube-modified ferrule graphite electrode

被引：34
|
作者：
Sharma, S. P. [1 ]
Tomar, L. N. S. [1 ]
Acharya, J. [1 ]
Chaturvedi, A. [1 ]
Suryanarayan, M. V. S. [1 ]
Jain, Rajeev [2 ]
机构：
[1] Def Res & Dev Estab, Synthet Chem Div, Gwalior 474002, MP, India
[2] Jiwaji Univ, Sch Studies Chem, Gwalior 474002, India
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2012年 / 166卷
关键词：
Acetylcholinesterase; Carboxylated single-walled carbon nanotube; Ferrule graphite electrode; Sarin; DFP; IMMOBILIZED ORGANOPHOSPHORUS HYDROLASE; COBALT PHTHALOCYANINE; ELECTROCHEMICAL BIOSENSORS; PASTE ELECTRODES; NERVE AGENTS; PESTICIDES; SENSOR; INSECTICIDE; RESIDUES; PARAOXON;
D O I：
10.1016/j.snb.2012.03.022
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this work, highly sensitive acetylcholinesterase (AChE) inhibition-based amperometric biosensor has been developed. AChE was immobilized by cross-linking with glutaraldehyde onto ferrule graphite electrode (FGE) modified with carboxylated single-walled carbon nanotube (cSWCNT). The properties of optimized biosensors are characterized by scanning electron microscopy (SEM), cyclic voltammograms (CVs) and electrochemical impedance spectroscopy (EIS). The synergistic action of cSWCNT/FGE showed excellent electrocatalytic activity with low applied potential for the detection of acetylthiocholine (ACTh). Sarin and diisopropylfluorophosphate (DFP) could be detected up to 25-35 nM and 15-20 nM respectively at 30-40% inhibition level of AChE and followed linearity in wide range concentration. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：616 / 623
页数：8
相关论文
共 50 条
  • [1] Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine
    Apetrei, Irina Mirela
    Apetrei, Constantin
    INTERNATIONAL JOURNAL OF NANOMEDICINE, 2013, 8 : 4391 - 4397
  • [2] Single-walled carbon nanotube-magnetite nanoparticles film modified ferrule graphite electrode for voltammetric determination of 2-diisopropylaminoethanethiol
    Sharma, S. P.
    Farukh, M.
    Chaturvedi, A.
    Srivastava, A. R.
    Suryanarayana, M. V. S.
    Jain, Rajeev
    SENSORS AND ACTUATORS B-CHEMICAL, 2012, 161 (01): : 414 - 421
  • [3] Acetylcholinesterase biosensor based on single-walled carbon nanotubes-Co phtalocyanine for organophosphorus pesticides detection
    Ivanov, A. N.
    Younusov, R. R.
    Evtugyn, G. A.
    Arduini, F.
    Moscone, D.
    Palleschi, G.
    TALANTA, 2011, 85 (01) : 216 - 221
  • [4] An amperometric biosensor based on acetylcholinesterase immobilized onto iron oxide nanoparticles/multi-walled carbon nanotubes modified gold electrode for measurement of organophosphorus insecticides
    Chauhan, Nidhi
    Pundir, Chandra Shekhar
    ANALYTICA CHIMICA ACTA, 2011, 701 (01) : 66 - 74
  • [5] A Sensitive Amperometric Acetylcholine Biosensor Based on Carbon Nanosphere and Acetylcholinesterase Modified Electrode for Detection of Pesticide Residues
    Jian-Rong Cai
    Li-Na Zhou
    En Han
    Analytical Sciences, 2014, 30 : 669 - 673
  • [6] A Sensitive Amperometric Acetylcholine Biosensor Based on Carbon Nanosphere and Acetylcholinesterase Modified Electrode for Detection of Pesticide Residues
    Cai, Jian-Rong
    Zhou, Li-Na
    Han, En
    ANALYTICAL SCIENCES, 2014, 30 (06) : 669 - 673
  • [7] Differential pulse voltammetric simultaneous determination of acetaminophen and ascorbic acid using single-walled carbon nanotube-modified carbon-ceramic electrode
    Habibi, Biuck
    Jahanbakhshi, Mojtaba
    Pournaghi-Azar, Mohammad Hossein
    ANALYTICAL BIOCHEMISTRY, 2011, 411 (02) : 167 - 175
  • [8] Acetylcholinesterase biosensor based on chitosan/ZnO nanocomposites modified electrode for amperometric detection of pesticides
    Guan, Huanan
    Jiang, Jialiang
    Chen, Dandan
    Wang, Wei
    Wang, Yan
    Xin, Jiaying
    PROCEEDINGS OF THE 2015 INTERNATIONAL CONFERENCE ON MATERIALS, ENVIRONMENTAL AND BIOLOGICAL ENGINEERING, 2015, 10 : 163 - 166
  • [9] Voltammetry Detection of Ascorbic Acid at Glassy Carbon Electrode Modified by Single-Walled Carbon Nanotube/Zinc Oxide
    Ngai, Koh Sing
    Tan, Wee Tee
    Zainal, Zulkarnain
    Zawawi, Ruzniza Mohd
    Zidan, Mohammed
    INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE, 2013, 8 (08): : 10557 - 10567
  • [10] An amperometric acetylcholinesterase sensor based on Fe3O4 nanoparticle/multi-walled carbon nanotube-modified ITO-coated glass plate for the detection of pesticides
    Chauhan, Nidhi
    Pundir, Chandra Shekhar
    ELECTROCHIMICA ACTA, 2012, 67 : 79 - 86
12345