Enhanced production of α-amylase by Penicillium chrysogenum in liquid culture by modifying the process parameters

In this paper, we have assessed the role of changing physicochemical parameters and substrate types on the production of alpha-amylase enzyme from Penicillium chrysogenum, with a view to determining the optimal conditions required for its maximum production. The findings of this research revealed that, at pH 6 using linseed oil cake as substratum, alpha-amylase enzyme production was maximum (550.0 U/mL), when the fungi was incubated for 6 days at 30 degrees C in 0.1 M acetate buffer. Further, reasonably good production of the alpha-amylase enzyme was also observed at pH 9 with all the experimented carbon sources as substrates. Moreover, statistical analysis, using analysis of variance (ANOVA) carried out to study the impact of different studied parameters on the alpha-amylase enzyme production revealed that incubation period of 6-18 days is highly significant (p = 0.01) factor in amylotic activity of the P. chrysogenum. Under the researched out optimal conditions, P. chrysogenum is an economically viable option for the industrial and biotechnological production of alpha-amylase enzyme. (C) 2015 Elsevier Ltd. All rights reserved.