A rapid and highly reliable field-based LAMP assay of canine parvovirus

被引：15

作者：

Parthiban, M. ^{[1
]}

Divya, Kurunchi C. ^{[1
]}

Kumanan, K. ^{[1
]}

Bargavi, D. S. ^{[1
]}

机构：

[1] Madras Vet Coll, Dept Anim Biotechnol, Madras 600007, Tamil Nadu, India

来源：

ACTA VIROLOGICA | 2012年 / 56卷 / 01期

关键词：

canine parvovirus; nested polymerase chain reaction; loop-mediated isothermal amplification; sensitivity; specificity; MEDIATED ISOTHERMAL AMPLIFICATION; POLYMERASE-CHAIN-REACTION; VIRUS;

D O I：

10.4149/av_2012_01_71

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Loop-mediated isothermal amplification (LAMP) is known as a rapid and reliable alternative to conventional single-step or nested PCR for detection of genomic DNA of various pathogens in clinical samples. In this study, LAMP assay was developed for canine parvovirus (CPV) and compared with single-step and nested PCR assays. Out of 50 fecal samples from dogs clinically suspected for CPV infections, 19 were found positive by single-step PCR, 22 by nested PCR and 26 by LAMP. LAMP products were subjected to restriction analysis and sequencing to check their specificity. LAMP assay turned out to be a rapid and fairly reproducible method, did not amplify other common canine pathogens and was more sensitive than nested PCR assay. Therefore, it can be regarded as a highly reliable method for routine field diagnosis of CPV infection.

引用

页码：71 / 74

页数：4

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