Pore Formation in Lipid Bilayer Membranes made of Phosphatidylcholine and Cholesterol Followed by Means of Constant Current

被引：23
|
作者：
Naumowicz, Monika [1 ]
Figaszewski, Zbigniew Artur [1 ,2 ]
机构：
[1] Univ Bialystok, Inst Chem, PL-15443 Bialystok, Poland
[2] Univ Warsaw, Fac Chem, Lab Electrochem Power Sources, PL-02093 Warsaw, Poland
来源：
CELL BIOCHEMISTRY AND BIOPHYSICS | 2013年 / 66卷 / 01期
关键词：
Chronopotentiometry; Electroporation; Lipid bilayer; Cholesterol; Capacitance; Resistance; ELECTRICAL BREAKDOWN; IMPEDANCE ANALYSIS; ALPHA-TOCOPHEROL; ELECTROPORATION; CAPACITANCE; SYSTEM; CELLS; MODEL;
D O I：
10.1007/s12013-012-9459-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper describes the application of chronopotentiometry to lipid bilayer research. The experiments were performed on bilayer lipid membranes composed of phosphatidylcholine and cholesterol and formed using the painting technique. Chronopotentiometric (U = f(t)) measurements were used to determine the membrane capacitance, resistance, and breakdown voltage as well as pore conductance and diameter.
引用
收藏
页码：109 / 119
页数：11
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