Laccase-assisted formation of bioactive chitosan/gelatin hydrogel stabilized with plant polyphenols

被引：94

作者：

Rocasalbas, Guillem ^{[1
]}

Francesko, Antonio ^{[1
]}

Tourino, Sonia ^{[1
]}

Fernandez-Francos, Xavier ^{[2
]}

Guebitz, Georg M. ^{[3
]}

Tzanov, Tzanko ^{[1
]}

机构：

[1] Univ Politecn Cataluna, Dept Chem Engn, Grp Mol & Ind Biotechnol, Terrassa 08222, Spain

[2] Univ Rovira & Virgili, Dept Analyt & Organ Chem, Tarragona 43007, Spain

[3] Graz Univ Technol, Inst Environm Biotechnol, A-8010 Graz, Austria

来源：

CARBOHYDRATE POLYMERS | 2013年 / 92卷 / 02期

关键词：

Chitosan; Chronic wounds; Gelatin; Laccase; Hydrogels; Phenolic compounds; HAMAMELIS-VIRGINIANA BARK; ANTIOXIDANT ACTIVITY; IN-VITRO; COLLAGENASE; INHIBITION; GELATIN; SKIN; MYELOPEROXIDASE; POLYMERIZATION; TYROSINASE;

D O I：

10.1016/j.carbpol.2012.10.045

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Laccase-assisted simultaneous cross-linking and functionalization of chitosan/gelatin blends with phenolic compounds from Hamamelis virginiana was investigated for the development of bioactive hydrogel dressings. The potential of these hydrogels for chronic wound treatment was evaluated in vitro, assessing their antibacterial and inhibitory effect on myeloperoxidase and collagenase. Rheological studies revealed that the mechanical properties of the hydrogels were a function of the enzymatic reaction time. Stable hydrogels and resistant to lysozyme degradation were achieved after 2 h laccase reaction. The inhibitory capacity of the hydrogel for myeloperoxidase and collagenase was 32% and 79% respectively after 24 h incubation. Collagenase activity was additionally suppressed by adsorption (20%) of the enzyme onto the hydrogel. Therefore, the bioactive properties of the hydrogels were due to the effect of both released phenolic compounds and the permanently functionalized platform itself. The hydrogels showed antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：989 / 996

页数：8

共 43 条

[1]

BRAND-WILLIAMS W, 1995, FOOD SCI TECHNOL-LEB, V28, P25

[2] Oxidation of guaiacol by myeloperoxidase:: a two-electron-oxidized guaiacol transient species as a mediator of NADPH oxidation [J].

Capeillère-Blandin, C .

BIOCHEMICAL JOURNAL, 1998, 336 :395-404

[3] Biomimetic surface modification of poly(L-lactic acid) with chitosan and its effects on articular chondrocytes in vitro [J].

Cui, YL ;

Di Qi, A ;

Liu, WG ;

Wang, XH ;

Wang, H ;

Ma, DM ;

De Yao, K .

BIOMATERIALS, 2003, 24 (21) :3859-3868

[4] Enzymatic polymerization of phenolic compounds using laccase and tyrosinase from Ustilago maydis [J].

Desentis-Mendoza, Rosa Martha ;

Hernandez-Sanchez, Humberto ;

Moreno, Abel ;

Emilio, Rojas del C. ;

Chel-Guerrero, Luis ;

Tamariz, Joaquin ;

Jaramillo-Flores, Maria Eugenia .

BIOMACROMOLECULES, 2006, 7 (06) :1845-1854

[5] High molecular compounds (polysaccharides and proanthocyanidins) from Hamamelis virginiana bark:: influence on human skin keratinocyte proliferation and differentiation and influence on irritated skin [J].

Deters, A ;

Dauer, A ;

Schnetz, E ;

Fartasch, M ;

Hensel, A .

PHYTOCHEMISTRY, 2001, 58 (06) :949-958

[6] Inhibition of deleterious chronic wound enzymes with plant polyphenols [J].

Diaz-Gonzalez, Maria ;

Rocasalbas, Guillem ;

Francesko, Antonio ;

Tourino, Sonia ;

Torres, Josep Lluis ;

Tzanov, Tzanko .

BIOCATALYSIS AND BIOTRANSFORMATION, 2012, 30 (01) :102-110

[7] AMINO ACID COMPOSITION OF MAMMALIAN COLLAGEN AND GELATIN [J].

EASTOE, JE .

BIOCHEMICAL JOURNAL, 1955, 61 (04) :589-600

[8] Nonrandom Distribution of Pseudomonas aeruginosa and Staphylococcus aureus in Chronic Wounds [J].

Fazli, Mustafa ;

Bjarnsholt, Thomas ;

Kirketerp-Moller, Klaus ;

Jorgensen, Bo ;

Andersen, Anders Schou ;

Krogfelt, Karen A. ;

Givskov, Michael ;

Tolker-Nielsen, Tim .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (12) :4084-4089

[9] Hypochlorous acid oxygenates the cysteine switch domain of pro-matrilysin (MMP-7). A mechanism for matrix metalloproteinase activation and atherosclerotic plaque rupture by myeloperoxidase. [J].

Fu, XY ;

Kassim, SY ;

Parks, WC ;

Heinecke, JW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (44) :41279-41287

[10] Protective Effect of Withania somnifera and Cardiospermum halicacabum Extracts Against Collagenolytic Degradation of Collagen [J].

Ganesan, Krishnamoorthy ;

Sehgal, Praveen Kumar ;

Mandal, Asit Baran ;

Sayeed, Sadulla .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2011, 165 (3-4) :1075-1091

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