Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes

被引:916
作者
Levasseur, Anthony [1 ]
Drula, Elodie [2 ]
Lombard, Vincent [2 ]
Coutinho, Pedro M. [2 ]
Henrissat, Bernard [2 ]
机构
[1] Aix Marseille Univ, ESIL Polytech Marseille, Biotechnol Champignons Filamenteux UMR1163, INRA, F-13288 Marseille 09, France
[2] Aix Marseille Univ, CNRS, UMR6098, F-13288 Marseille 09, France
关键词
CAZy database; Evolution of lignocellulose breakdown; Ligninolytic enzymes; Lytic polysaccharide monooxygenases; GLYCOSIDE HYDROLASE FAMILY; CELLOBIOSE DEHYDROGENASE; PHANEROCHAETE-CHRYSOSPORIUM; ALCOHOL OXIDASE; GMC OXIDOREDUCTASES; TRICHODERMA-REESEI; FUNGAL LACCASE; LIGNIN; DEGRADATION; CLASSIFICATION;
D O I
10.1186/1754-6834-6-41
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Since its inception, the carbohydrate-active enzymes database (CAZy; www.cazy.org) has described the families of enzymes that cleave or build complex carbohydrates, namely the glycoside hydrolases (GH), the polysaccharide lyases (PL), the carbohydrate esterases (CE), the glycosyltransferases (GT) and their appended noncatalytic carbohydrate-binding modules (CBM). The recent discovery that members of families CBM33 and family GH61 are in fact lytic polysaccharide monooxygenases (LPMO), demands a reclassification of these families into a suitable category. Results: Because lignin is invariably found together with polysaccharides in the plant cell wall and because lignin fragments are likely to act in concert with (LPMO), we have decided to join the families of lignin degradation enzymes to the LPMO families and launch a new CAZy class that we name "Auxiliary Activities" in order to accommodate a range of enzyme mechanisms and substrates related to lignocellulose conversion. Comparative analyses of these auxiliary activities in 41 fungal genomes reveal a pertinent division of several fungal groups and subgroups combining their phylogenetic origin and their nutritional mode (white vs. brown rot). Conclusions: The new class introduced in the CAZy database extends the traditional CAZy families, and provides a better coverage of the full extent of the lignocellulose breakdown machinery.
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页数:14
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