Docetaxel-loaded nanoparticles based on star-shaped mannitol-core PLGA-TPGS diblock copolymer for breast cancer therapy

被引：119
作者：
Tao, Wei [1 ,2 ,3 ]
Zeng, Xiaowei [1 ,2 ,3 ]
Liu, Ting [4 ]
Wang, Zhongyuan [1 ,2 ,3 ]
Xiong, Qingqing [5 ,6 ]
Ouyang, Chunping [7 ]
Huang, Laiqiang [1 ,2 ,3 ]
Mei, Lin [1 ,2 ,3 ]
机构：
[1] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Ctr Biotech & Biomed, Shenzhen Key Lab Gene & Antibody Therapy, Shenzhen 518055, Peoples R China
[3] Tsinghua Univ, Div Life & Hlth Sci, Grad Sch Shenzhen, Shenzhen 518055, Peoples R China
[4] Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China
[5] Peking Union Med Coll, Inst Biomed Engn, Tianjin 300192, Peoples R China
[6] Chinese Acad Med Sci, Tianjin Key Lab Biomat Res, Tianjin 300192, Peoples R China
[7] Kingfa Sci & Technol Co Ltd, Ctr Res & Dev, Guangzhou 510520, Guangdong, Peoples R China
来源：
ACTA BIOMATERIALIA | 2013年 / 9卷 / 11期
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
Star-shaped copolymer; Docetaxel; Nanoparticles; Drug delivery; Nanotechnology for cancer therapy; VITAMIN-E TPGS; ORAL DELIVERY; CONTROLLED-RELEASE; ANTICANCER DRUGS; WATER; FORMULATIONS; MICELLES; EFFICACY; SIZE; PCL;
D O I：
10.1016/j.actbio.2013.06.034
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
A star-shaped biodegradable polymer, mannitol-core poly(D,L-lactide-co-glycolide)-D-alpha-tocopheryl polyethylene glycol 1000 succinate (M-PLGA-TPGS), was synthesized in order to provide a novel nanoformulation for breast cancer chemotherapy. This novel copolymer was prepared by a core-first approach via three stages of chemical reaction, and was characterized by nuclear magnetic resonance, gel permeation chromatography and thermogravimetric analysis. The docetaxel-loaded M-PLGA-TPGS nanoparticles (NPs), prepared by a modified nanoprecipitation method, were observed to be near-spherical shape with narrow size distribution. Confocal laser scanning microscopy showed that the uptake level of M-PLGA-TPGS NPs was higher than that of PLGA NPs and PLGA-TPGS NPs in MCF-7 cells. A significantly higher level of cytotoxicity was achieved with docetaxel-loaded M-PLGA-TPGS NPs than with commercial Taxotere, docetaxel-loaded PLGA-TPGS and PLGA NPs. Examination of the drug loading and encapsulation efficiency proved that star-shaped M-PLGA-TPGS could carry higher levels of drug than linear polymer. The in vivo experiment showed docetaxel-loaded M-PLGA-TPGS NPs to have the highest anti-tumor efficacy. In conclusion, the star-like M-PLGA-TPGS copolymer shows potential as a promising drug-loaded biomaterial that can be applied in developing novel nanoformulations for breast cancer therapy. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：8910 / 8920
页数：11
相关论文
共 45 条
[1]   Rehydrated Lyophilized Rifampicin-Loaded mPEG-DSPE Formulations for Nebulization [J].
Abdulla, Juma Masoud Abdulla ;
Tan, Yvonne Tze-Fung ;
Darwis, Yusrida .
AAPS PHARMSCITECH, 2010, 11 (02) :663-671
[2]   Preparation of drug-loaded microspheres of linear and star-shaped poly(D,L-lactide)s and their drug release behaviors [J].
Baimark, Yodthong ;
Srisa-ard, Mangkorn .
JOURNAL OF APPLIED POLYMER SCIENCE, 2012, 124 (05) :3871-3878
[3]   Synthesis and characterisation of star branched polyesters with dendritic cores and the effect of structural variations on zero shear rate viscosity [J].
Claesson, H ;
Malmström, E ;
Johansson, M ;
Hult, A .
POLYMER, 2002, 43 (12) :3511-3518
[4]   Poly(lactide)-vitamin E derivative/montmorillonite nanoparticle formulations for the oral delivery of Docetaxel [J].
Feng, Si-Shen ;
Mei, Lin ;
Anitha, Panneerselvan ;
Gan, Chee Wee ;
Zhou, Wenyou .
BIOMATERIALS, 2009, 30 (19) :3297-3306
[5]  
Grzegorz L., 2009, Prog. Polym. Sci, V34, P852
[6]  
Grzegorz L, 2009, POLYMER, V50, P77
[7]   Nanoformulation of D-α-tocopheryl polyethylene glycol 1000 succinate-b-poly(ε-caprolactone-ran-glycolide) diblock copolymer for breast cancer therapy [J].
Huang, Laiqiang ;
Chen, Hongbo ;
Zheng, Yi ;
Song, Xiaosong ;
Liu, Ranyi ;
Liu, Kexin ;
Zeng, Xiaowei ;
Mei, Lin .
INTEGRATIVE BIOLOGY, 2011, 3 (10) :993-1002
[8]  
Jeffrey AH, 2012, SCIENCE, V337, P303
[9]   A Pharmacodynamic Study of Docetaxel in Combination with the P-glycoprotein Antagonist Tariquidar (XR9576) in Patients with Lung, Ovarian, and Cervical Cancer [J].
Kelly, Ronan J. ;
Draper, Deborah ;
Chen, Clara C. ;
Robey, Robert W. ;
Figg, William D. ;
Piekarz, Richard L. ;
Chen, Xiaohong ;
Gardner, Erin R. ;
Balis, Frank M. ;
Venkatesan, Aradhana M. ;
Steinberg, Seth M. ;
Fojo, Tito ;
Bates, Susan E. .
CLINICAL CANCER RESEARCH, 2011, 17 (03) :569-580
[10]   Magnetic field activated lipid-polymer hybrid nanoparticles for stimuli-responsive drug release [J].
Kong, Seong Deok ;
Sartor, Marta ;
Hu, Che-Ming Jack ;
Zhang, Weizhou ;
Zhang, Liangfang ;
Jin, Sungho .
ACTA BIOMATERIALIA, 2013, 9 (03) :5447-5452
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