Complementary methods for contact hypersensitivity (CHS) evaluation in mice

被引：18

作者：

Zemelka-Wiacek, Magdalena ^{[1
]}

Majewska-Szczepanik, Monika ^{[1
]}

Pyrczak, Wieslaw ^{[2
]}

Szczepanik, Marian ^{[1
]}

机构：

[1] Jagiellonian Univ, Dept Med Biol, Coll Med, PL-31034 Krakow, Poland

[2] Jagiellonian Univ, Ctr Comp Sci, Coll Med, PL-31034 Krakow, Poland

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2013年 / 387卷 / 1-2期

关键词：

Contact hypersensitivity; DNFB; Ear-swelling; Vascular permeability; MPO; IFN-gamma; DELAYED-HYPERSENSITIVITY; ADAPTIVE IMMUNITY; MECHANISMS; CELLS; ELICITATION; INITIATION; ANTIGEN; MEDIATE; INNATE;

D O I：

10.1016/j.jim.2012.11.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Contact hypersensitivity (CHS) is an experimental model of allergic contact dermatitis (ACD) that can be studied in mice. For CHS responses, mice are immunized by painting with a reactive hapten, such as 1-fluoro-4,6-dinitrobenzene (DNFB), on the shaved abdominal and chest skin. Subsequently, the ears are challenged with diluted hapten, eliciting 'hypersensitive' ear-swelling reactions, which can be measured with a micrometer. In this manuscript we present complementary methods that can be used to evaluate CHS in mice that include: ear weight vascular permeability, myeloperoxidase (MPO) activity, IFN-gamma concentration in ear extracts and also IFN-gamma production by auricular lymph node cells (ELNC). The biochemical evaluation of CHS can be also supported by proliferation assay, measurement of IFN-gamma production by skin-draining lymph node cells employing ELISA test and by evaluation of IFN-gamma(+) TCR alpha beta CD8 cells with the use of flow cytometry. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：270 / 275

页数：6

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