Heparan sulfate-mediated binding of infectious dengue virus type 2 and yellow fever virus

被引：188
作者：
Germi, R
Crance, JM
Garin, D
Guimet, J
Lortat-Jacob, H
Ruigrok, RWH
Zarski, JP
Drouet, E
机构：
[1] CRSSA Emile Parde, Unite Virol, F-38702 La Tronche, France
[2] Univ Grenoble 1, Fac Med, Lab Virol Mol & Struct, EA 2939, F-38700 La Tronche, France
[3] CNRS, CEA, UJF,UMR 5075, Inst Biol Struct, F-38027 Grenoble 1, France
[4] EMBL, Grenoble Outstn, F-38042 Grenoble 9, France
[5] CHU Grenoble, Dept Gastroenterol Hepatol, F-38043 Grenoble, France
来源：
VIROLOGY | 2002年 / 292卷 / 01期
关键词：
flaviviruses; heparan sulfate; receptor; binding; glycosaminoglycan;
D O I：
10.1006/viro.2001.1232
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Dengue virus type 2 and Yellow fever virus are arthropod-borne flaviviruses causing hemorrhagic fever in humans. Identification of virus receptors is important in understanding flavivirus pathogenesis. The aim of this work was to study the role of cellular heparan sulfate in the adsorption of infectious Yellow fever and Dengue type 2 viruses. Virus attachment was assessed by adsorbing virus to cells, washing unbound virus away, releasing cell-bound virus by freezing/thawing, and then titrating the released infectious virus. Treatment of cells by heparin-lyase, desulfation of cellular heparan sulfate, or treatment of the virus with heparin inhibited cell binding of both viruses. Heparin also inhibited Yellow fever virus infection by 97%. Using infectious virus, the present work shows the importance of heparan sulfate in binding and infection of these two flaviviruses. (C) 2002 Elsevier Science.
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收藏
页码：162 / 168
页数：7
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