Effect of bradykinin on TGF-β1-induced retinal pigment epithelial cell proliferation and extracellular matrix secretion

被引：7

作者：

Cai, Wenting ^{[1
]}

Wei, Qingquan ^{[2
]}

Liu, Qingyu ^{[1
]}

Ren, Chengda ^{[1
]}

Liu, Junling ^{[1
]}

Zhang, Ruiling ^{[1
]}

He, Mengmei ^{[1
]}

Wang, Qianyi ^{[1
]}

Du, Yaru ^{[3
]}

Yu, Jing ^{[1
]}

机构：

[1] Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Ophthalmol, Shanghai, Peoples R China

[2] Nanchang Univ, Nanchang, Jiangxi, Peoples R China

[3] 180th Hosp Chinese Peoples Liberat Army, Dept Ophthalmol, Quanzhou, Fujian Province, Peoples R China

来源：

BMC OPHTHALMOLOGY | 2016年 / 16卷

基金：

中国国家自然科学基金;

关键词：

Proliferative vitreoretinopathy; Retinal pigment epithelium; Bradykinin; Transforming growth factor-beta 1; Extracellular matrix; TGF-BETA; SUBRETINAL MEMBRANES; METALLOPROTEINASES; DETACHMENT; TGF-BETA-2; EXPRESSION; PROTEINS; DISEASE; FLUID;

D O I：

10.1186/s12886-016-0373-3

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Background: To evaluate the effect of bradykinin (BK) on TGF-beta 1-induced retinal pigment epithelial (RPE) cell proliferation and extracellular matrix secretion and to elucidate the relationship between BK and the Erk/Akt signaling pathway. Methods: The effects of BK on TGF-beta 1-induced RPE cell proliferation were examined via CCK-8 assay. Cell culture supernatant collagen I concentrations were measured via ELISA. Fibronectin (Fn), matrix metalloproteinase-2 (MMP-2) and MMP-9 mRNA and protein expression levels were measured via q-PCR and Western blotting, respectively. Changes in Akt/Erk phosphorylation induced by BK and HOE-140 were evaluated via Western blotting. Results: TGF-beta 1 stimulated ARPE-19 cell proliferation, which was inhibited by BK, whose effects were inhibited by HOE-140. BK inhibited TGF-beta 1-induced collagen I, Fn and MMP-2 secretion in RPE cells, and these effects were inhibited by HOE-140. BK also inhibited TGF-beta 1-induced Akt phosphorylation in RPE cells, and these effects were blocked by HOE-140. BK had no significant effect on Erk-mediated signaling. Conclusions: The findings from this study indicate that BK could be novel therapeutic targets for the treatment of PVR.

引用

页码：1 / 9

页数：9

共 35 条

[1] GROWTH-FACTORS IN VITREOUS AND SUBRETINAL FLUID CELLS FROM PATIENTS WITH PROLIFERATIVE VITREORETINOPATHY [J].

BAUDOUIN, C ;

FREDJREYGROBELLET, D ;

BRIGNOLE, F ;

NEGRE, F ;

LAPALUS, P ;

GASTAUD, P .

OPHTHALMIC RESEARCH, 1993, 25 (01) :52-59

[2] Bradykinin inhibits high glucose- and growth factor-induced collagen synthesis in mesangial cells through the B2-kinin receptor [J].

Blaes, Nelly ;

Pecher, Christiane ;

Mehrenberger, Marion ;

Cellier, Eric ;

Praddaude, Francoise ;

Chevalier, Jacques ;

Tack, Ivan ;

Couture, Rejean ;

Girolami, Jean-Pierre .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2012, 303 (02) :F293-F303

[3] Proliferative vitreoretinopathy: A new concept of disease pathogenesis and practical consequences [J].

Carlos Pastor, J. ;

Rojas, Jimena ;

Pastor-Idoate, Salvador ;

Di Lauro, Salvatore ;

Gonzalez-Buendia, Lucia ;

Delgado-Tirado, Santiago .

PROGRESS IN RETINAL AND EYE RESEARCH, 2016, 51 :125-155

[4]

Ciprian Danielescu, 2015, Rom J Ophthalmol, V59, P88

[5] CORRELATION OF FIBROSIS AND TRANSFORMING GROWTH FACTOR-BETA TYPE-2 LEVELS IN THE EYE [J].

CONNOR, TB ;

ROBERTS, AB ;

SPORN, MB ;

DANIELPOUR, D ;

DART, LL ;

MICHELS, RG ;

DEBUSTROS, S ;

ENGER, C ;

KATO, H ;

LANSING, M ;

HAYASHI, H ;

GLASER, BM .

JOURNAL OF CLINICAL INVESTIGATION, 1989, 83 (05) :1661-1666

[6] TGF-β1 Induced Transdifferentiation of RPE Cells is Mediated by TAK1 [J].

Dvashi, Zeev ;

Goldberg, Mordechai ;

Adir, Orit ;

Shapira, Michal ;

Pollack, Ayala .

PLOS ONE, 2015, 10 (04)

[7] Role of retinal detachment subretinal fluid on extracellular matrix metabolism [J].

González-Avila, G ;

Méndez, D ;

Lozano, D ;

Ramos, C ;

Delgado, J ;

Iturria, C .

OPHTHALMOLOGICA, 2004, 218 (01) :49-56

[8] Pathobiology of epiretinal and subretinal membranes: possible roles for the matricellular proteins thrombospondin 1 and osteonectin (SPARC) [J].

Hiscott, P ;

Hagan, S ;

Heathcote, L ;

Sheridan, CM ;

Groenewald, CP ;

Grierson, I ;

Wong, D ;

Paraoan, L .

EYE, 2002, 16 (04) :393-403

[9] Stimulation of integrin-mediated cell contractility by fibronectin polymerization [J].

Hocking, DC ;

Sottile, J ;

Langenbach, KJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (14) :10673-10682

[10] Lutein acts via multiple antioxidant pathways in the photo-stressed retina [J].

Kamoshita, Mamoru ;

Toda, Eriko ;

Osada, Hideto ;

Narimatsu, Toshio ;

Kobayashi, Saori ;

Tsubota, Kazuo ;

Ozawa, Yoko .

SCIENTIFIC REPORTS, 2016, 6

← 1 2 3 4 →