The store-operated calcium current I-CRAC: Nonlinear activation by InsP(3) and dissociation from calcium release

被引：200

作者：

Parekh, AB ^{[1
]}

Fleig, A ^{[1
]}

Penner, R ^{[1
]}

机构：

[1] MAX PLANCK INST BIOPHYS CHEM, D-37077 GOTTINGEN, GERMANY

来源：

CELL | 1997年 / 89卷 / 06期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/S0092-8674(00)80282-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Patch-clamp experiments aimed at determining the relationship between intracellular Ca2+ release and activation of store-operated calcium current I-CRAC reveal that both agonist and InsP(3)-mediated activation of I-CRAC are highly nonlinear, occurring over a narrow concentration range. Ca2+ release and Ca2+ influx can be dissociated, as they possess differential sensitivities to InsP(3): low concentrations induce substantial Ca2+ release without any activation of I-CRAC, whereas micromolar concentrations of InsP(3) are required to activate Ca2+ influx. This suggests functionally distinct stores controlling Ca2+ release and influx and enables cells to switch between sources of Ca2+ to fit best their current needs.

引用

页码：973 / 980

页数：8

共 29 条

[1]

ALI H, 1990, J BIOL CHEM, V265, P745

[2] ANTIGEN AND THAPSIGARGIN PROMOTE INFLUX OF CA2+ IN RAT BASOPHILIC RBL-2H3 CELLS BY OSTENSIBLY SIMILAR MECHANISMS THAT ALLOW FILLING OF INOSITOL 1,4,5-TRISPHOSPHATE-SENSITIVE AND MITOCHONDRIAL CA2+ STORES [J].

ALI, H ;

MAEYAMA, K ;

SAGIEISENBERG, R ;

BEAVEN, MA .

BIOCHEMICAL JOURNAL, 1994, 304 :431-440

[3]

Berridge Michael J., 1992, Advances in Second Messenger and Phosphoprotein Research, V26, P211

[4] INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].

BERRIDGE, MJ .

NATURE, 1993, 361 (6410) :315-325

[5] ACTIVATION OF CA2+ ENTRY INTO ACINAR-CELLS BY A NON-PHOSPHORYLATABLE INOSITOL TRISPHOSPHATE [J].

BIRD, GS ;

ROSSIER, MF ;

HUGHES, AR ;

SHEARS, SB ;

ARMSTRONG, DL ;

PUTNEY, JW .

NATURE, 1991, 352 (6331) :162-165

[6]

CHIAVAROLI C, 1994, J BIOL CHEM, V269, P25570

[7] CAPACITATIVE CA2+ ENTRY EXCLUSIVELY INHIBITS CAMP SYNTHESIS IN C6-2B GLIOMA-CELLS - EVIDENCE THAT PHYSIOLOGICALLY EVOKED CA2+ ENTRY REGULATES CA2+-INHIBITABLE ADENYLYL-CYCLASE IN NONEXCITABLE CELLS [J].

CHIONO, M ;

MAHEY, R ;

TATE, G ;

COOPER, DMF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (03) :1149-1155

[8]

CHOI OH, 1993, J IMMUNOL, V151, P5586

[9]

DEMAUREX N, 1992, J BIOL CHEM, V267, P2318

[10] P(2Y) PURINOCEPTORS IN NORMAL NIH 3T3 AND IN NIH 3T3 OVEREXPRESSING C-RAS [J].

GIOVANNARDI, S ;

RACCA, C ;

BERTOLLINI, L ;

STURANI, E ;

PERES, A .

EXPERIMENTAL CELL RESEARCH, 1992, 202 (02) :398-404

← 1 2 3 →