Double-membrane gap junction internalization requires the clathrin-mediated endocytic machinery

被引:63
作者
Gumpert, Anna M. [1 ]
Varco, Joseph S. [1 ]
Baker, Susan M. [1 ]
Piehl, Michelle [1 ]
Falk, Matthias M. [1 ]
机构
[1] Lehigh Univ, Dept Biol Sci, Bethlehem, PA 18015 USA
关键词
annular gap junction; clathrin; connexin; endocytosis; gap junction; RNAi;
D O I
10.1016/j.febslet.2008.07.024
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Direct cell-cell communication mediated by plasma membrane-spanning gap junction (GJ) channels is vital to all aspects of cellular life. Obviously, GJ intercellular communication (GJIC) requires precise regulation, and it is known that controlled biosynthesis and degradation, and channel opening and closing (gating) are exploited. We discovered that cells internalize GJs in response to various stimuli. Here, we report that GJ internalization is a clathrin-mediated endocytic process that utilizes the vesicle-coat protein clathrin, the adaptor proteins adaptor protein complex 2 and disabled 2, and the GTPase dynamin. To our knowledge, we are first to report that the endocytic clathrin machinery can internalize double-membrane vesicles into cells. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码:2887 / 2892
页数:6
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