Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources

被引：9

作者：

Yuan, Bo ^{[1
,2
]}

Wang, Shi-An ^{[1
,3
]}

Li, Fu-Li ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100039, Peoples R China

[3] Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 10期

关键词：

Biofuel; Ethanol; Inulinase; Jerusalem Artichoke; Yeast; JERUSALEM-ARTICHOKE;

D O I：

10.1007/s10529-013-1251-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To improve inulin utilization and ethanol fermentation, exoinulinase genes from the yeast Kluyveromyces marxianus and the recently identified yeast, Candida kutaonensis, were expressed in Saccharomyces cerevisiae. S. cerevisiae harboring the exoinulinase gene from C. kutaonensis gave higher ethanol yield and productivity from both inulin (0.38 vs. 0.34 g/g and 1.35 vs. 1.22 g l(-1) h(-1)) and Jerusalem artichoke tuber flour (0.47 vs. 0.46 g/g and 1.62 vs. 1.54 g l(-1) h(-1)) compared with the strain expressing the exoinulinase gene from K. marxianus. Thus, the exoinulinase gene from C. kutaonensis is advantageous for engineering S. cerevisiae to improve ethanol fermentation from inulin sources.

引用

页码：1589 / 1592

页数：4

共 8 条

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