S-Nitrosothiols, Nitric Oxide and Proinflammatory Cytokines in Children with Inflammatory Bowel Disease

Background: The aim of the study was to investigate the levels of S-nitrosothiols (RSNO) in blood serum of children with inflammatory bowel disease (IBD), nonspecific ulcerative colitis (NUC) and Crohn's disease (CD), and correlate the obtained data with the number of total nitric oxide metabolites (NOx) and proinflammatory cytokines (PC). Methods: RSNO and NOx were determined by the Griess method and PC by ELISA. We examined 174 children of both genders aged from 6 to 17 years (mean age - 14 years) including 112 children suffering from IBD and 62 children from the control group. The results were statistically processed using Statistica 6.1. Results: The obtained data show an increased but statistically indistinguishable RSNO number in NUC and CD. We revealed the absence of correlation between RSNO and NO and unidirectional RSNO and PC values. Conclusions: The obtained data of RSNO concentrations in blood serum of children with IBD suggest the participation of RSNO in IBD pathogenesis in children and an indirect correlation of the translation of nitric oxide synthase aberrant activity into RSNO.