Chemiluminescence Determination of Gossypol by Oxidation with N-Bromosuccinimide

被引：0

作者：

Han, Suqin ^{[1
]}

Hao, Fang ^{[1
]}

Wei, Bei ^{[1
]}

机构：

[1] Shanxi Normal Univ, Sch Chem & Mat Sci, Linfen 041004, Peoples R China

来源：

JOURNAL OF THE CHINESE CHEMICAL SOCIETY | 2013年 / 60卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Chemiluminescence; Gossypol; N-Bromosuccinimide; PERFORMANCE LIQUID-CHROMATOGRAPHY; CANCER-CELLS; COTTONSEED; SPECTROSCOPY; APOPTOSIS; FLUIDS;

D O I：

10.1002/jccs.201200464

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A chemiluminescence (CL) phenomenon was observed when gossypol was injected into a reaction mixture of N-bromosuccinimide (NBS) and alkaline dichlorofluorescein (DCF). Based on this phenomenon, a rapid and sensitive method for the determination of gossypol was established. Under the optimum conditions, the linear range was from 1.0 x 10(-9) to 1.0 x 10(-6) M. The detection limit was 1.0 x 10(-10) M. The method has been applied to the determination of gossypol in cottonseed, cottonseed oil, pharmaceutical and biological fluids with satisfactory results. The possible CL reaction mechanism was discussed briefly.

引用

页码：1070 / 1074

页数：5

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