Optimizing alginate beads for the immobilisation of Phaeodactylum tricornutum in estuarine waters

被引：14

作者：

Cabrita, Maria Teresa ^{[1
]}

Raimundo, Joana ^{[1
]}

Pereira, Patricia ^{[1
]}

Vale, Carlos ^{[1
]}

机构：

[1] IPMA, P-1449006 Lisbon, Portugal

来源：

MARINE ENVIRONMENTAL RESEARCH | 2013年 / 87-88卷

关键词：

Cell immobilisation; Phaeodactylum tricornutum; Hardening gel agent; Monitoring tool; Water quality; Estuary; TAGUS ESTUARY; PHYTOPLANKTON; INHIBITION; MICROALGA; STABILITY; SEDIMENTS; DIATOMS; STATE;

D O I：

10.1016/j.marenvres.2013.03.002

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study addresses the influence of calcium as hardening agent, on alginate gel bead stability and suitability for the growth of Phaeodactylum tricornutum Bohlin (Bacillariophyceae) in estuarine waters. Alginate beads produced with 1, 2, 4, 5 and 6% of CaCl2 solutions were investigated for stability and suitability for growth of P. tricornutum cells, under mean salinity 27, at 220 and 440 rpm stirring laboratory conditions, and in devices placed under in situ estuarine conditions. Gel stability and suitability for cell growth were evaluated through bead diameter, bead disruption, dissolution and loss of spherical shape, cell viability and specific growth rates. Beads gelled with 5% CaCl2 were found the most suitable to sustain gel stability and cell growth in the estuarine waters. These beads were surveyed during dredging operations in the Tagus estuary, both in situ and in estuarine water under laboratory conditions, showing significantly lowered growth rates possibly due to Mn, Co and As accumulated in the cells. Results confirmed that the monitoring tool presented is reliable and effective for the assessment of anthropogenic impacts. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：37 / 43

页数：7

共 39 条

[21] The effect of Ni2+, Co2+, Zn2+, Cd2+ and Hg2+ on the growth rate of marine diatom Phaeodactylum tricornutum bohlin:: Microplate growth inhibition test [J].