The Belt and Road Initiative is one of China's greatest international economic directions, aiming at economic development in Asia, Africa and Europe, which are the main economic members all over the world. We analyze the developing background of the Belt and Road Initiative, combining with the initial function of customs and the appeal with the Belt and Road Initiative and analyzing the collaboration developing problems they face. Current situation of customs confronting are analyzed from the perspective of mutual infrastructure construction, international cooperation, and holding summit forums among different countries. Previous experience of EUCU could be used for reference on the customs cooperation. The customs cooperation could be an important booster for the development among related countries. However, the collaboration of customs also face many barriers, such as different political policies of different countries, lack of uniformed policies and contracts.