Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components

被引：162

作者：

Holcik, M

Liebhaber, SA

机构：

[1] UNIV PENN,SCH MED,DEPT GENET,HOWARD HUGHES MED INST,PHILADELPHIA,PA 19104

[2] UNIV PENN,SCH MED,DEPT MED,HOWARD HUGHES MED INST,PHILADELPHIA,PA 19104

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 06期

关键词：

D O I：

10.1073/pnas.94.6.2410

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Assembly of a sequence-specific RNA-protein complex on the 3' untranslated region (3'UTR) of human alpha-globin mRNA (alpha-complex) correlates with mRNA stabilization, Here we map a limited segment of the alpha-globin 3'UTR that is both necessary and sufficient for alpha-complex formation. The sequence of this binding region identifies three additional, highly stable mRNAs that share closely related, pyrimidine-rich cis-motifs in their respective 3'UTRs. Each mRNA assembles a sequence-specific ribonucleoprotein complex at this conserved region, These complexes are structurally related, and each contains a 39-kDa cytoplasmic poly(C) binding protein previously demonstrated to be essential to formation of the alpha-complex, These observations indicate the existence of a general determinant for stabilization of eukaryotic mRNAs.

引用

页码：2410 / 2414

页数：5

共 24 条

[1] TISSUE-SPECIFIC EXPRESSION AND CDNA STRUCTURE OF A HUMAN TRANSCRIPT ENCODING A NUCLEIC-ACID BINDING [OLIGO(DC)] PROTEIN RELATED TO THE PRE-MESSENGER-RNA BINDING-PROTEIN-K [J].