Localization of Entamoeba histolytica Amebopore in Amebic Liver Abscesses in Hamsters

被引：4
作者：
Gonzalez, A.
Monterrubio, D.
Nequiz, M.
Lopez, R.
Olivos, A.
Leon, C. Garcia de
Tello, E.
Salaiza, N.
Ramos, E.
Gudino, M.
Montfort, I.
Perez-Tamayo, R.
机构：
[1] Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
[2] Gen Hosp, Mexico City 06726, DF, Mexico
来源：
ANIMAL BIODIVERSITY AND EMERGING DISEASES: PREDICTION AND PREVENTION | 2008年 / 1149卷
关键词：
Entamoeba histolytica; amebopore; amebic liver abscesses;
D O I：
10.1196/annals.1428.006
中图分类号：
Q14 [生态学（生物生态学）];
学科分类号：
071012 ; 0713 ;
摘要：
Amebopore was purified from axenically grown trophozoites of the Entamoeba histolytica strain HM1:IMSS. The purification procedure involved Mono Q anion-exchange chromatography and electroelution. Sequence analysis of the final product revealed that amebopore A was completely pure. Polyclonal antibodies against the purified amebopore were obtained from rabbits, and Western blot studies demonstrated their specificity. Sections of experimental, acute (1, 2, 3, and 4 days), amebic liver abscesses produced in hamsters were stained with the anti-amebopore antibody; in all the analyzed stages, amebopore appeared as a constitutively expressed cytoplasmic molecule in trophozoites. No extracellular or hepatocyte-membrane amebopore was found. This study is the first to trace amebopore in an in vivo model of amebic liver abscesses.
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收藏
页码：375 / 379
页数：5
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