Characterization of Bombyx mori nucleopolyhedrovirus with a deletion of bm118

被引：11

作者：

Yang, Zhang-Nv ^{[1
]}

Xu, Hai-Jun ^{[1
]}

Park, Enoch Y. ^{[2
]}

Zhang, Chuan-Xi ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Insect Sci, Hangzhou 310029, Zhejiang, Peoples R China

[2] Shizuoka Univ, Grad Sch Sci & Technol, Integrated Biosci Sect, Suruga Ku, Shizuoka 4228529, Japan

来源：

VIRUS RESEARCH | 2008年 / 135卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Bombyx mori; nucleopolyhedrovirus; ORF118; BV production; nucleocapsids maturation;

D O I：

10.1016/j.virusres.2008.03.016

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bombyx mori nucleopolyhedrovirus (BmNPV) ORF118 (bm118) is homologous to Autographa californica nucleopolyhedrovirus (AcMNPV) ORF142, one of the core genes existing in all baculovirus genomes sequenced to date, suggesting that Bm118 plays a critical role in viral infection. In this study, the primary role of Bm118 was investigated by using homologous recombination in Escherichia coli to generate a bm118 knockout bacmid containing the BmNPV genome. In addition, the bm118 rescue bacmid was constructed by transposing a bm118 gene cassette into the polh locus of the bm118 knockout bacmid. Transfection assays demonstrated that the bm118 knockout bacmid was incapable of producing budded virion (BV). Nevertheless, this defect could be partially recovered by a rescue bacmid. Electron microscopy analysis revealed that the bm118 knockout produced aberrant capsids characterized by translucent, elongated nucleocapsids present as bundles within the nuclei. This construct also produced polyhedra lacking virions. These results reveal that Bm118 is essential for BV production and nucelocapsid maturation. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：220 / 229

页数：10

共 31 条

[1]

Adams J.R., 1991, P87

[2] BACULOVIRUS DIVERSITY AND MOLECULAR-BIOLOGY [J].

BLISSARD, GW ;

ROHRMANN, GF .

ANNUAL REVIEW OF ENTOMOLOGY, 1990, 35 :127-155

[3] Determination of the protein composition of the occlusion-derived virus of Autographa californica nucleopolyhedrovirus [J].

Braunagel, SC ;

Russell, WK ;

Rosas-Acosta, G ;

Russell, DH ;

Summers, MD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (17) :9797-9802

[4] AN ELECTRON-MICROSCOPE STUDY OF PARTIALLY LYSED BACULOVIRUS NUCLEOCAPSIDS - THE INTRA-NUCLEOCAPSID PACKAGING OF VIRAL-DNA [J].

BUD, HM ;

KELLY, DC .

JOURNAL OF ULTRASTRUCTURE RESEARCH, 1980, 73 (03) :361-368

[5] Autographa californica multiple nucleopolyhedrovirus exon0 (orf141), which encodes a RING finger protein, is required for efficient production of budded virus [J].

Dai, XJ ;

Stewart, TM ;

Pathakamuri, JA ;

Li, QJ ;

Theilmann, DA .

JOURNAL OF VIROLOGY, 2004, 78 (18) :9633-9644

[6] One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].

Datsenko, KA ;

Wanner, BL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645

[7] Characterization of a nucleopolyhedrovirus with a deletion of the baculovirus core gene Bm67 [J].

Ge, Jun-Qing ;

Yang, Zhang-Nv ;

Tang, Xu-Dong ;

Xu, Hai-Jun ;

Hong, Jian ;

Chen, Jian-Guo ;

Zhang, Chuan-Xi .

JOURNAL OF GENERAL VIROLOGY, 2008, 89 :766-774

[8] Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus [J].

Gomi, S ;

Majima, K ;

Maeda, S .

JOURNAL OF GENERAL VIROLOGY, 1999, 80 :1323-1337

[9] EARLY EVENTS IN INFECTION OF HELIOTHISZEA MIDGUT CELLS BY A BACULOVIRUS [J].

GRANADOS, RR .

VIROLOGY, 1978, 90 (01) :170-174

[10] The genome sequence and evolution of baculoviruses [J].

Herniou, EA ;

Olszewski, JA ;

Cory, JS ;

O'Reilly, DR .

ANNUAL REVIEW OF ENTOMOLOGY, 2003, 48 :211-234

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