Protective effect of glycine supplementation on the levels of lipid peroxidation and antioxidant enzymes in the erythrocyte of rats with alcohol-induced liver injury

被引：52

作者：

Senthilkumar, R ^{[1
]}

Sengottuvelan, M ^{[1
]}

Nalini, N ^{[1
]}

机构：

[1] Annamalai Univ, Dept Biochem, Annamalainagar 608002, Tamil Nadu, India

来源：

CELL BIOCHEMISTRY AND FUNCTION | 2004年 / 22卷 / 02期

关键词：

antioxidants; erythrocyte membrane; ethanol; glycine; lipid peroxidation;

D O I：

10.1002/cbf.1062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the effect of glycine supplementation on lipid peroxidation and antioxidants in the erythrocyte membrane, plasma and hepatocytes of rats with alcohol-induced hepatotoxicity. Administering ethanol (20%) for 60 days to male Wistar rats resulted in significantly elevated levels of erythrocyte membrane, plasma and hepatocyte thiobarbituric acid reactive substances (TBARS) as compared with those of the experimental control rats. Decreased activities of superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione peroxidase (GPx) and glutathione reductase (GR) were also observed on alcohol supplementation as compared with those of the experimental control rats. Glycine was administered at a dose of 0.6 g kg(-1) body weight to rats with alcohol-induced liver injury, which significantly decreased the levels of TBARS and significantly elevated the activities of SOD, CAT, GSH, GPx and GR in the erythrocyte membrane, plasma and hepatocytes as compared to that of untreated alcohol supplemented rats. Thus, our data indicate that supplementation with glycine offers protection against free radical-mediated oxidative stress in the erythrocyte membrane, plasma and hepatocytes of animals with alcohol-induced liver injury. Copyright (C) 2003 John Wiley Sons, Ltd.

引用

页码：123 / 128

页数：6

共 34 条

[11]

FERNANDEZCHECA J, 1992, HEPATIC ANION TRANSP, P363

[12] GSH transport in mitochondria: Defense against TNF-induced oxidative stress and alcohol-induced defect [J].

FernandezCheca, JC ;

Kaplowitz, N ;

GarciaRuiz, C ;

Colell, A ;

Miranda, M ;

Mari, M ;

Ardite, E ;

Morales, A .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1997, 273 (01) :G7-G17

[13] BIOLOGICAL EFFECTS OF THE SUPEROXIDE RADICAL [J].

FRIDOVICH, I .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1986, 247 (01) :1-11

[14] FREE-RADICALS, ANTIOXIDANTS, AND HUMAN-DISEASE - CURIOSITY, CAUSE, OR CONSEQUENCE [J].

HALLIWELL, B .

LANCET, 1994, 344 (8924) :721-724

[15]

HARRIS RA, 1986, TXB BIOCH, P293

[16]

HEBBEL RP, 1986, J LAB CLIN MED, V107, P401

[17]

HEBBEL RP, 1984, BLOOD, V64, P733

[18] THE EFFECT OF MALONYLDIALDEHYDE, A PRODUCT OF LIPID-PEROXIDATION, ON THE DEFORMABILITY, DEHYDRATION AND CR-51-SURVIVAL OF ERYTHROCYTES [J].

JAIN, SK ;

MOHANDAS, N ;

CLARK, MR ;

SHOHET, SB .

BRITISH JOURNAL OF HAEMATOLOGY, 1983, 53 (02) :247-255

[19]

JERNSTROM B, 1993, BASIC LIFE SCI, V61, P137

[20]

KAKKAR P, 1984, INDIAN J BIOCHEM BIO, V21, P130

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