I5M:: 3D widefield light microscopy with better than 100 nm axial resolution

被引:0
作者
Gustafsson, MGL
Agard, DA [1 ]
Sedat, JW
机构
[1] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA
关键词
cytoskeleton; deconvolution; (IM)-M-2; (IM)-M-5; (IM)-M-n; interference; microtubule; optical microscopy; OTF; resolution; superresolution; widefield microscopy;
D O I
暂无
中图分类号
TH742 [显微镜];
学科分类号
摘要
Sevenfold improved axial resolution has been achieved in three-dimensional widefield fluorescence microscopy, using a novel interferometric technique in which the sample is observed and/or illuminated from both sides simultaneously using two opposing objective lenses. Separate interference effects in the excitation light and the emitted light give access to higher resolution axial information about the sample than can be reached by conventional widefield or confocal microscopes. Here we report the experimental verification of this resolution performance on complex biological samples.
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页码:10 / 16
页数:7
相关论文
共 13 条
  • [1] Abbe E., 1873, ARCH MIKROSK ANAT, V9, P413, DOI DOI 10.1007/BF02956173
  • [2] AGARD DA, 1989, METHOD CELL BIOL, V30, P353
  • [3] ENHANCEMENT OF AXIAL RESOLUTION IN FLUORESCENCE MICROSCOPY BY STANDING-WAVE EXCITATION
    BAILEY, B
    FARKAS, DL
    TAYLOR, DL
    LANNI, F
    [J]. NATURE, 1993, 366 (6450) : 44 - 48
  • [4] GUSTAFSSON MGL, 1995, P SOC PHOTO-OPT INS, V2412, P147, DOI 10.1117/12.205334
  • [5] 3D widefield microscopy with two objective lenses: Experimental verification of improved axial resolution
    Gustafsson, MGL
    Agard, DA
    Sedat, JW
    [J]. THREE-DIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING III, 1996, 2655 : 62 - 66
  • [6] PROPERTIES OF A 4PI CONFOCAL FLUORESCENCE MICROSCOPE
    HELL, S
    STELZER, EHK
    [J]. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1992, 9 (12) : 2159 - 2166
  • [7] Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range
    Hell, SW
    Schrader, M
    VanderVoort, HTM
    [J]. JOURNAL OF MICROSCOPY, 1997, 187 : 1 - 7
  • [8] Lanni F., 1993, Bioimaging, V1, P187, DOI 10.1002/1361-6374(199312)1:4<187::AID-BIO1>3.3.CO
  • [9] 2-G
  • [10] Pawley J. B., 1990, HDB BIOL CONFOCAL MI