Callogenesis and Shoot Organogenesis from Flowers of Stevia rebaudiana (Bert.)

被引:30
作者
Ahmad, Nisar [1 ,2 ]
Fazal, Hina [3 ]
Zamir, Roshan [2 ]
Khalil, Shahid Akbar [2 ]
Abbasi, Bilal Haider [1 ]
机构
[1] Quaid I Azam Univ, Fac Biol Sci, Dept Biotechnol, Islamabad 45320, Pakistan
[2] Nucl Inst Food & Agr, Tarnab Peshawar 25000, Pakistan
[3] Pakistan Council Sci & Ind Res, Peshawar 25000, Pakistan
关键词
Stevia rebaudiana; Callogenesis; Shoot organogenesis; Flowers; Palynology;
D O I
10.1007/s12355-011-0083-3
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The callogenic and shoot organogenic potential of the medicinaly important plant, S. rebaudiana were investigated. Callus induction and shoot regeneration were induced from flower explants of greenhouse plants incubated on Murashige and Skoog (MS)-medium supplemented with different plant growth regulators (PGRs). The best callogenic response was observed on explants incubated on MS-medium supplemented with 2.0 mg/l 6-benzyladenine (BA) along with 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) after 30-days of culture. Subsequent transfer of callogenic explants onto MS-medium supplemented with 2.0 mg/l BA gave 85.2% shoot organogenesis after 30-days following culture. Maximum number (21.6) of shoots/explant was recorded for 2.0 mg/l BA. Subsequent sub culturing of shoots after 3 weeks of culture, on medium with similar compositions of PGRs gave 5.1 cm longest shoot. The palynology of S. rebaudiana has also been investigated.
引用
收藏
页码:174 / 177
页数:4
相关论文
共 20 条
[1]   Echinacea biotechnology:: Challenges and opportunities [J].
Abbasi, Bilal Haider ;
Saxena, Praveen K. ;
Murch, Susan J. ;
Liu, Chun-Zhao .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2007, 43 (06) :481-492
[2]   Shoot regeneration and free-radical scavenging activity in Silybum marianum L. [J].
Abbasi, Bilal Haider ;
Khan, Mubarak Ali ;
Mahmood, Tariq ;
Ahmad, Mushtaq ;
Chaudhary, Muhammad Fayyaz ;
Khan, Mir Ajab .
PLANT CELL TISSUE AND ORGAN CULTURE, 2010, 101 (03) :371-376
[3]   Efficient regeneration and antioxidant potential in regenerated tissues of Piper nigrum L. [J].
Ahmad, Nisar ;
Fazal, Hina ;
Abbasi, Bilal Haider ;
Rashid, Muhammad ;
Mahmood, Tariq ;
Fatima, Nighat .
PLANT CELL TISSUE AND ORGAN CULTURE, 2010, 102 (01) :129-134
[4]  
[Anonymous], 2005, SUGAR TECH, DOI DOI 10.1007/BF02942413
[5]  
[Anonymous], 1952, POLLEN MORPHOLOGY PL
[6]  
Hayat MQ, 2010, PAK J BOT, V42, P751
[7]   Rapid in vitro propagation and enhanced stevioside accumulation in Stevia rebaudiana Bert [J].
Hwang, Sung Jin .
JOURNAL OF PLANT BIOLOGY, 2006, 49 (04) :267-270
[8]   Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana [J].
Ibrahim I.A. ;
Nasr M.I. ;
Mohammed B.R. ;
El-Zefzafi M.M. .
Sugar Tech, 2008, 10 (3) :254-259
[9]  
Ibrahim I.A., 2008, SUGAR TECH, V10, P148
[10]  
Joint FAO/ WHO Expert Committee on Food Additives, 2006, WHO FOOD ADDIT SER, V54, P117