FenF:: Servicing the mycosubtilin synthetase assembly line in trans

被引：18

作者：

Aron, Zachary D. ^{[1
]}

Fortin, Pascal D. ^{[1
]}

Calderone, Christopher T. ^{[1
]}

Walsh, Christopher T. ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

CHEMBIOCHEM | 2007年 / 8卷 / 06期

关键词：

biosynthesis; enzymes; kinetics; mycosubtilin; polyketides; transferases;

D O I：

10.1002/cbic.200600575

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

AT your service. We report the expression and characterization of FenF from mycosubtilin biosynthesis. This work represents the first kinetic and selectivity studies performed on an in trans AT domain servicing a polyketide synthase (PKS), and revealed a strong acyl-group specificity and broad promiscuity toward substrate carrier proteins. The lack of specificity in FenF-mediated malonyl transfer suggests that this protein might prove a powerful tool for combinatorial biosynthesis. (Chemical Equation Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：613 / 616

页数：4

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