Synovial hyperplasia in HTLV-I associated arthropathy is induced by tumor necrosis factor-α produced by HTLV-I infected CD68+cells

被引：0

作者：

Yin, WH ^{[1
]}

Hasunuma, T ^{[1
]}

Kobata, T ^{[1
]}

Sumida, T ^{[1
]}

Nishioka, K ^{[1
]}

机构：

[1] St Marianna Univ, Sch Med, Inst Med Sci, Rheumatol Immunol & Genet Program,Miyamae Ku, Kawasaki, Kanagawa 2168512, Japan

来源：

JOURNAL OF RHEUMATOLOGY | 2000年 / 27卷 / 04期

关键词：

synovial hyperplasia; HTLV-I; tumor necrosis factor-alpha; CD68+cell;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. To investigate the pathogenic role of macrophage Lineage (CD68+) cells in synovial proliferation in patients with human T cell leukemia virus I (HTLV-I) associated arthropathy (HAAP). Methods. Synovial tissues obtained from 3 patients with HAAP and 3 patients with osteoarthritis (OA) were examined for the expression of tumor necrosis factor-alpha (TNF-alpha) mRNA, HTLV-I tax/rex mRNA, and number of CD68 by in situ reverse transcription assay and immunohistochemistry. Western blot and flow cytometric analyses were used to determine TNF-alpha production in HTLV-I infected synoviocytes. Changes in CD68+ cell population were examined by flow cytometric analysis. Proliferative effects of supernatants of HTLV-I infected synoviocytes on normal synoviocytes were also determined. Results. TNF-alpha and HTLV-I tax/rex mRNA were preferentially expressed in CD68+ cells in HAAP synovia. Infection of OA synoviocytes by HTLV-I resulted in preferential expression in CD68+ cells, and these cells produced TNF-alpha. Supernatants of HTLV-I infected synoviocytes significantly enhanced the proliferation of normal synoviocytes through a TNF-alpha, dependent pathway. Conclusion, Our results suggest HTLV-I viral tropism for CD68+ cells, and that HTLV-I infected synoviocytes were induced to produce TNF-alpha, which enhances synovial proliferation in HAAP.

引用

页码：874 / 881

页数：8