High-Frequency Ultrasound Assessment of the Murine Heart From Embryo Through to Juvenile

被引：33

作者：

Corrigan, Niamh ^{[1
]}

Brazil, Derek P.

Auliffe, Fionnuala M. ^{[1
]}

机构：

[1] Univ Coll Dublin, Natl Matern Hosp, UCD Obstet & Gynaecol, Dublin 2, Ireland

来源：

REPRODUCTIVE SCIENCES | 2010年 / 17卷 / 02期

基金：

爱尔兰科学基金会;

关键词：

High frequency ultrasound; cardiac function; embryonic murine heart; POSTNATAL-DEVELOPMENT; MOUSE; ECHOCARDIOGRAPHY; PATTERNS; MICE;

D O I：

10.1177/1933719109348923

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Aim: The aim of this study is to assess the murine heart of normal embryos, neonates, and juveniles using high-frequency ultrasound. Methods: Diastolic function was measured with E/A ratio (E wave velocity/A wave velocity) and isovolumetric relaxation time (IRT), systolic function with isovolumetric contraction time (ICT), percentage fractional shortening (FS%), percentage ejection fraction (EF%). Global cardiac performance was quantified using myocardial performance index (MPI). Results: Isovolumetric relaxation time remained stable from E10.5 to 3 weeks. Systolic function (ICT) improved with gestation and remained stable from E18.5 onward. Myocardial performance index showed improvement in embryonic lift (0.82-0.63) and then stabilized from 1 to 3 week (0.60-0.58). Percentage ejection fraction remained high during gestation (77%-69%) and then decreased from the neonate to juvenile (68%-51%). Conclusion: The ultrasound biomicroscope allows for noninvasive in-depth assessment of cardiac function of embryos and pups. Detailed physiological and functional cardiac function readouts can be obtained, which is invaluable for comparison to mouse models of disease.

引用

页码：147 / 157

页数：11

共 24 条

[1] Initial experience with high frequency ultrasound for the newborn C57BL mouse [J].

Bose, Alok K. ;

Mathewson, James W. ;

Anderson, Brent E. ;

Andrews, Allyson M. ;

Gerdes, A. Martin ;

Perryman, M. Benjamin ;

Grossfeld, Paul D. .

ECHOCARDIOGRAPHY-A JOURNAL OF CARDIOVASCULAR ULTRASOUND AND ALLIED TECHNIQUES, 2007, 24 (04) :412-419

[2] Doppler echocardiography of normal and abnormal embryonic mouse heart [J].

Gui, YH ;

Linask, KK ;

Khowsathit, P ;

Huhta, JC .

PEDIATRIC RESEARCH, 1996, 40 (04) :633-642

[3] Mouse heart valve structure and function: echocardiographic and morphometric analyses from the fetus through the aged adult [J].

Hinton, Robert B., Jr. ;

Alfieri, Christina M. ;

Witt, Sandra A. ;

Glascock, Betty J. ;

Khoury, Philip R. ;

Benson, D. Woodrow ;

Yutzey, Katherine E. .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2008, 294 (06) :H2480-H2488

[4] Embryonic and neonatal phenotyping of genetically engineered mice [J].

Kulandavelu, S ;

Qu, DW ;

Sunn, N ;

Mu, JW ;

Rennie, MY ;

Whiteley, KJ ;

Walls, JR ;

Bock, NA ;

Sun, JCH ;

Covelli, A ;

Sled, JG ;

Adamson, SL .

ILAR JOURNAL, 2006, 47 (02) :103-117

[5]

Leatherbury Linda, 2003, Birth Defects Research, V69, P83, DOI 10.1002/bdrc.10005

[6] Baf60c is essential for function of BAF chromatin remodelling complexes in heart development [J].

Lickert, H ;

Takeuchi, JK ;

von Both, I ;

Walls, JR ;

McAuliffe, F ;

Adamson, SL ;

Henkelman, RM ;

Wrana, JL ;

Rossant, J ;

Bruneau, BG .

NATURE, 2004, 432 (7013) :107-112

[7] Progressive troponin I loss impairs cardiac relaxation and causes heart failure in mice [J].

Liu, Jing ;

Du, Jianfeng ;

Zhang, Chi ;

Walker, Jeffery W. ;

Huang, Xupei .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2007, 293 (02) :H1273-H1281

[8] Human fetal cardiac function during the first trimester of pregnancy [J].

Mäkikallio, K ;

Jouppila, P ;

Räsänen, J .

HEART, 2005, 91 (03) :334-338

[9] Fgl2 deficiency causes neonatal death and cardiac dysfunction during embryonic and postnatal development in mice [J].

Mu, Junwu ;

Qu, Dawei ;

Bartczak, Agata ;

Phillips, M. James ;

Manuel, Justin ;

He, Wei ;

Koscik, Cheryl ;

Mendicino, Michael ;

Zhang, Li ;

Clark, David A. ;

Grant, David R. ;

Backx, Peter H. ;

Levy, Gary A. ;

Adamson, S. Lee .

PHYSIOLOGICAL GENOMICS, 2007, 31 (01) :53-62

[10] 40 MHz Doppler characterization of umbilical and dorsal aortic blood flow in the early mouse embryo [J].

Phoon, CKL ;

Aristizabal, O ;

Turnbull, DH .

ULTRASOUND IN MEDICINE AND BIOLOGY, 2000, 26 (08) :1275-1283

← 1 2 3 →