Kaposi's sarcoma-associated herpesvirus open reading frame 50/Rta protein activates the entire viral lytic cycle in the HH-B2 primary effusion lymphoma cell line

被引:235
作者
Gradoville, L
Gerlach, J
Grogan, E
Shedd, D
Nikiforow, S
Metroka, C
Miller, G
机构
[1] Yale Univ, Sch Med, Dept Pediat, New Haven, CT 06520 USA
[2] Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
[3] Yale Univ, Sch Med, Dept Epidemiol & Publ Hlth, New Haven, CT 06520 USA
[4] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06520 USA
[5] Yale Univ, Sch Med, Dept Immunol, New Haven, CT 06520 USA
[6] St Lukes Roosevelt Hosp Ctr, New York, NY USA
关键词
D O I
10.1128/JVI.74.13.6207-6212.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Rta, the gene product of Kaposi's sarcoma-associated herpesvirus (KSHV) encoded mainly in open reading frame 50 (ORF50), is capable of activating expression of viral lytic cycle genes. What was not demonstrated in previous studies was whether KSHV Rta was competent to initiate the entire viral lytic life cycle including lytic viral DNA replication, late-gene expression with appropriate kinetics, and virus release. in HH-B2, a newly established primary effusion lymphoma (PEL) cell line, KSHV ORF50 behaved as an immediate-early gene and autostimulated its own expression. Expression of late genes, ORF65, and K8.1 induced by KSHV Rta was eliminated by phosphonoacetic acid, an inhibitor of viral DNA polymerase. Transfection of KSHV Rta increased the production of encapsidated DNase-resistant viral DNA from HH-BZ cells. Thus, introduction of an ORF50 expression plasmid is sufficient to drive the lytic cycle to completion in cultured PEL cells.
引用
收藏
页码:6207 / 6212
页数:6
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