Quantitative profiles of the mRNAs of ER-α and its novel variant ER-α36 in breast cancers and matched normal tissues

被引:19
|
作者
Zheng, Yi [1 ,2 ]
Zhang, Jing [1 ,2 ]
Xu, Zhen-zhen [1 ]
Sheng, Jian-ming [2 ]
Zhang, Xiao-chen [2 ]
Wang, Hao-hao [1 ,2 ]
Teng, Xiao-dong [3 ]
Liu, Xiao-jiao [4 ]
Cao, Jiang [1 ]
Teng, Li-song [2 ]
机构
[1] Zhejiang Univ, Sir Run Run Shaw Inst Clin Med, Key Lab Biotherapy Zhejiang Prov, Hangzhou 310016, Zhejiang, Peoples R China
[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Ctr Canc, Hangzhou 310003, Zhejiang, Peoples R China
[3] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Pathol, Hangzhou 310003, Zhejiang, Peoples R China
[4] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Gen Surg, Hangzhou 310003, Zhejiang, Peoples R China
来源
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B | 2010年 / 11卷 / 02期
基金
中国国家自然科学基金;
关键词
Breast cancer; Estrogen receptor; Estrogen receptor-alpha 36 (ER-alpha 36); ER-alpha; 66; ESTROGEN-RECEPTOR-ALPHA; AFRICAN-AMERICAN WOMEN; TUMORS; EXPRESSION; IDENTIFICATION; ISOFORM; ACTIVATION; PROGNOSIS; GENE; BETA;
D O I
10.1631/jzus.B0900266
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The novel estrogen receptor-alpha (ER-alpha) variant ER-alpha 36 is reported to be functional in the estrogen signaling pathway and is related to tamoxifen resistance in breast cancer. However, ER-alpha 36 tends to be a favorable factor for survival in patients without tamoxifen therapy. To investigate the mechanisms behind this paradox, we determined the differences between the transcriptional profiles of ER-alpha 36 and full-length ER-alpha (ER-alpha 66) in breast cancers and matched normal tissues. We analyzed ER-alpha 36 and ER-alpha 66 messenger RNA (mRNA) levels in 74 pairs of breast cancers and matched normal tissues using a real-time quantitative polymerase chain reaction (PCR) assay, and correlated the results with their clinicopathological characteristics. Breast cancers expressed lower ER-alpha 36 mRNA levels than matched normal tissues regardless of their ER-alpha 66 expression status. Down-regulation of ER-alpha 36 mRNA was correlated with local progression, lymph node metastasis, and advanced cancer stage. The level of ER-alpha 66 mRNA was lower in ER-alpha negative breast cancers compared with matched normal tissues. No differences in ER-alpha 66 mRNA levels were observed during cancer progression. Down-regulation of ER-ga36 is associated with carcinogenesis and progression of breast cancer.
引用
收藏
页码:144 / 150
页数:7
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