Pyridinium trifluoroacetate/N-methylimidazole as an efficient activator for oligonucleotide synthesis via the phosphoramidite method

被引：26
作者：
Eleuteri, A [1 ]
Capaldi, DC [1 ]
Krotz, AH [1 ]
Cole, DL [1 ]
Ravikumar, VT [1 ]
机构：
[1] ISIS Pharmaceut, Dev Chem, Carlsbad, CA 92008 USA
来源：
ORGANIC PROCESS RESEARCH & DEVELOPMENT | 2000年 / 4卷 / 03期
关键词：
D O I：
10.1021/op9900378
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A new activator is reported for coupling phosphoramidites to a free 5'-hydroxyl group during oligonucleotide synthesis. Pyridinium trifluoroacetate/N-methyl imidazole is a remarkably efficient replacement for 1H-tetrazole in the solid-supported synthesis of oligonucleotides, This reagent is safe and inexpensive, is not moisture-sensitive, and is soluble in acetonitrile.
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页码：182 / 189
页数：8
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