Screening for anti-infective properties of several medicinal plants of the Mauritians flora

被引:62
作者
Rangasamy, Ournadevi
Raoelison, Guy
Rakotoniriana, Francisco E.
Cheuk, Kiban
Urverg-Ratsimamanga, Suzanne
Quetin-Leclercq, Joelle
Gurib-Fakim, Ameenah
Subratty, Anwar Hussein [1 ]
机构
[1] Univ Mauritius, Fac Sci, Dept Hlth Sci, Reduit, Mauritius
[2] IMRA, Dept Phytochem & Microbiol, Antananarivo 101, Madagascar
[3] Catholic Univ Louvain, Lab Pharmacognosie, Unite CHAM, B-1200 Brussels, Belgium
[4] Univ Mauritius, Fac Sci, Dept Chem, Reduit, Mauritius
关键词
medicinal plants; Mauritius; infections; bioautography; anti-microbial activity; micro-dilution assay;
D O I
10.1016/j.jep.2006.08.002
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Several plants of the Mauritian flora alleged to possess anti-infective properties were studied against different strains of pathogenic bacteria and fungi. The grounded dried plant materials were extracted with different extractants and screened for anti-microbial activity using the disk diffusion and the micro-dilution techniques. Preliminary screening revealed that the methanol extracts were most active. Salmonella enteritidis, Enterobacter cloacae and Bacillus subtilis were the three test organisms, which were found to be susceptible to all the crude methanolic extracts of the different plants investigated (100% susceptibility), followed by Escherichia coli (57.1%) and Pseudomonas aeruginosa (57.1%), and Staphylococcus aureus (28.6%). The lowest minimum inhibitory concentration recorded for the different crude methanol extracts against Staphylococcus aureus, Escherichia coli, Salmonella enteritidis, Enterobacter cloacae, Bacillus subtilis and the mould fungus Candida albicans were 500, 1000, 125, 250, 1000 and 125 mu g/ml, respectively. Bioautography using Cladosporium cucumerinum revealed that dichloromethane (DCM) extracts had the highest activity against the phytopathogenic fungus. It was also noted that the DCM extracts of Michelia champaca and Antidesma madagascariense yielded the maximum number of growth inhibiting compounds against Cladosporium cucumerinum. Activity of the different crude extracts was also investigated against several phytopathogenic filamentous fungi, Colletotrichum glocosporoides, Rhizoctonia solani, Sclerotinia sclerotium, Guignardia sp. and Fusarium oxysporurn. It was found that crude hexane extracts as well as crude DCM extracts exhibited marked activity against several strains of fungi, especially Colletotrichum glocosporoides, Sclerotinia sclerotium and Guignardia sp. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:331 / 337
页数:7
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